尿酸酶在接枝于聚丙烯腈膜上的导电聚苯胺刷上的可逆固定化。
Reversible immobilization of uricase on conductive polyaniline brushes grafted on polyacrylonitrile film.
机构信息
Biochemical Processing and Biomaterial Research Laboratory, Department of Chemistry and Biology, Faculty of Arts and Sciences, Gazi University, Teknikokullar, Ankara, Turkey.
出版信息
Bioprocess Biosyst Eng. 2011 Feb;34(2):127-34. doi: 10.1007/s00449-010-0453-3. Epub 2010 Jul 21.
Polyacrylonitrile film (PAN) surfaces were modified with chemical polymerization of conductive polyaniline (PANI) in the presence of potassium dichromate as an oxidizing agent. The conductive films were used for immobilization of uricase. The surface resistance of the conductive film in this work was found to be 0.97 kΩ/cm. The maximum amount of immobilized enzyme on conductive film containing 2.4% PANI was about 216 μg/cm(2). The optimum pH for free and immobilized enzymes was observed at 7.0 and 7.5, respectively. The K (m) values for free and immobilized uricase were found to be 94 and 138 μM, respectively. V (max) values were calculated as 1.87 and 1.63 U/mg protein for the free and immobilized enzymes, respectively. Immobilized uricase exhibited ~68% of its original activity even after 2 months of storage at 4 °C while the free enzyme lost its initial activity within 4 weeks.
聚丙烯腈薄膜（PAN）表面经化学聚合方法修饰，在重铬酸钾存在的条件下聚合生成导电聚苯胺（PANI）。将所得的导电膜用于固定化尿酸酶。实验中发现，该导电膜的表面电阻为 0.97 kΩ/cm。在含有 2.4%PANI 的导电膜上固定化酶的最大量约为 216 μg/cm(2)。游离酶和固定化酶的最适 pH 值分别为 7.0 和 7.5。游离和固定化尿酸酶的 K(m)值分别为 94 和 138 μM。游离酶和固定化酶的 V(max)值分别计算为 1.87 和 1.63 U/mg 蛋白。固定化尿酸酶在 4°C 下储存 2 个月后仍保持其原始活性的 68%左右，而游离酶在 4 周内就失去了初始活性。
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