聚吡咯接枝聚丙烯腈导电复合膜上葡萄糖氧化酶的可逆固定化。
Reversible immobilization of glucose oxidase on polyaniline grafted polyacrylonitrile conductive composite membrane.
机构信息
Gazi University, Faculty of Arts and Sciences, Biochemical Processing and Biomaterial Research Laboratory, 06500 Teknikokullar, Ankara, Turkey.
出版信息
Bioresour Technol. 2010 Sep;101(18):6881-7. doi: 10.1016/j.biortech.2010.04.025. Epub 2010 May 4.
Polyacrylonitrile membrane surfaces were modified with chemical polymerization of conductive polyaniline. The effect of aniline concentration on the grafting efficiency was investigated. As the amount of grafted PANI increased on the PAN membrane, the electrical resistance of composite membrane decreased. Composite (PAN/PANI)-1-5 membrane were used for reversible immobilization of GOD. The immobilization efficiency and the activity of the immobilized GOD were increased with increasing PANI contents of the composite membrane. The maximum amount of immobilized enzyme into composite PAN/PANI-5 membrane containing 2.1% PANI was about 251 microg/cm(2). Immobilized GOD exhibited 66% of its original activity even after two months of storage at 4 degrees C while the free enzyme lost its initial activity after three weeks.
聚丙烯腈膜表面通过化学聚合的方法进行了导电聚苯胺的修饰。研究了苯胺浓度对接枝效率的影响。随着 PAN 膜上接枝 PANI 的量增加，复合膜的电阻降低。复合（PAN/PANI）-1-5 膜用于 GOD 的可逆固定化。随着复合膜中 PANI 含量的增加，固定化酶的效率和活性增加。固定化酶的最大量约为 251 μg/cm(2)，进入含有 2.1% PANI 的复合 PAN/PANI-5 膜中。固定化 GOD 即使在 4°C 下储存两个月后仍保留其原始活性的 66%，而游离酶在三周后失去初始活性。
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