3Bs Research Group-Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Guimarães, Portugal.
J Tissue Eng Regen Med. 2011 Feb;5(2):104-11. doi: 10.1002/term.294.
Bone tissue engineering success strongly depends on our ability to develop new materials combining osteoconductive, osteoinductive and osteogenic properties. Recent studies suggest that biomaterials incorporating silanol (Si-OH) groups promote and maintain osteogenesis. The purpose of the present research work was to provide evidence that using wet-spinning technologies and a calcium silicate solution as a coagulation bath, it was possible to develop an in situ functionalization methodology to obtain 3D wet-spun fibre meshes with Si-OH groups, through a simple, economic and reliable process. SPCL (blend of starch with polycaprolactone) fibre meshes were produced by wet-spinning, using a calcium silicate solution as a non-solvent and functionalized in situ with Si-OH groups. In vitro tests, using goat bone marrow stromal cells (GBMSCs), showed that SPCL-Si scaffolds sustained cell viability and proliferation. Furthermore, high ALP activity and matrix production indicated that Si-OH groups improve cellular functionality towards the osteoblastic phenotype. Using this methodology, and assembling several wet-spun fibre meshes, 3D meshes can be developed, aiming at designing osteoconductive/osteoinductive 3D structures capable of stimulating bone ingrowth in vivo.
骨组织工程的成功在很大程度上取决于我们开发新材料的能力，这些新材料应兼具骨诱导、骨传导和骨生成特性。最近的研究表明，含有硅醇（Si-OH）基团的生物材料可以促进和维持成骨作用。本研究工作的目的是提供证据，证明使用湿法纺丝技术和硅酸钙溶液作为凝固浴，可以通过简单、经济和可靠的工艺，开发一种原位功能化方法，获得具有 Si-OH 基团的 3D 湿法纺丝纤维网。使用硅酸钙溶液作为非溶剂，通过湿法纺丝制备了 SPCL（淀粉与聚己内酯的混合物）纤维网，并在原位进行 Si-OH 基团功能化。使用山羊骨髓基质细胞（GBMSCs）进行的体外测试表明，SPCL-Si 支架能够维持细胞活力和增殖。此外，高碱性磷酸酶（ALP）活性和基质生成表明 Si-OH 基团可改善细胞向成骨表型的功能。通过使用这种方法，并组装多个湿法纺丝纤维网，可以开发 3D 纤维网，旨在设计具有骨传导/骨诱导特性的 3D 结构，以刺激体内骨长入。
