Li Qiang, Xia Ya-Yi, Tang Ji-Cun, Wang Ri-Ying, Bei Chao-Yong, Zeng Yanjum
Department of Orthopedics, the Affiliated Hospital of Guilin Medical College, Guilin, Guangxi Zhuang Autonomous Region, China.
Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):137-42. doi: 10.3109/10731199.2010.502880. Epub 2010 Jul 23.
To investigate the biocompatibility of diamond-like carbon (DLC) coated nickel-titanium shape memory alloy (NiTi SMA) in vitro and in vivo.
The in vitro study was carried out by co-culturing the DLC coated and uncoated NiTi SMA with bone marrow mesenchymal stem cells (MSCs), respectively, and the in vivo study was carried out by fixing the rabbits' femoral fracture model by DLC coated and uncoated NiTi SMA embracing fixator for 4 weeks, respectively. The concentration of the cells, alkaline phosphatase (AKP), and nickel ion in culture media were detected, respectively, at the first to fifth day after co-culturing. The inorganic substance, osteocalcin, alkaline phosphatase (ALP), and tumor necrosis factor (TNF) in callus surrounding fracture and the Ni(+) in muscles surrounding fracture site, liver and brain were detected 4 weeks postoperatively.
The in vitro study showed that the proliferation of MSCs and the expression of AKP in the DLC-coated group were higher than the uncoated group (P < 0.05), while the uncoated group released more Ni(2+) into the culture media than that in the coated group (P < 0.05). The in vivo study revealed that the inorganic substance and AKP, osteocalcin, and TNF expression were significantly higher in the DLC coated NiTi SMA embracing fixator than that in the uncoated group (P < 0.05). Ni(2+) in liver, brain, and muscles surrounding the fracture were significantly lower in the DLC coated groups than that in the uncoated group (P < 0.05).
Nickel-titanium shape memory alloy coated by diamond-like carbon appears to have better biocompatibility in vitro and in vivo compared to the uncoated one.
研究类金刚石碳（DLC）涂层镍钛形状记忆合金（NiTi SMA）的体外和体内生物相容性。
体外研究分别将涂覆和未涂覆DLC的NiTi SMA与骨髓间充质干细胞（MSCs）共培养；体内研究分别用涂覆和未涂覆DLC的NiTi SMA环抱式固定器固定兔股骨骨折模型4周。共培养后第1至5天分别检测培养基中细胞浓度、碱性磷酸酶（AKP）和镍离子浓度。术后4周检测骨折周围骨痂中的无机物、骨钙素、碱性磷酸酶（ALP）和肿瘤坏死因子（TNF），以及骨折部位周围肌肉、肝脏和大脑中的镍离子（Ni(+)）。
体外研究显示，DLC涂层组MSCs的增殖和AKP的表达高于未涂层组（P < 0.05），而未涂层组向培养基中释放的Ni(2+)比涂层组更多（P < 0.05）。体内研究表明，DLC涂层NiTi SMA环抱式固定器组的无机物、AKP、骨钙素和TNF表达明显高于未涂层组（P < 0.05）。DLC涂层组骨折周围肝脏、大脑和肌肉中的Ni(2+)明显低于未涂层组（P < 0.05）。
与未涂层的镍钛形状记忆合金相比，类金刚石碳涂层的镍钛形状记忆合金在体外和体内似乎具有更好的生物相容性。
