Li Qiang, Zhang Qin, Sun Zhengyi
Institute of Orthopaedics, Second Hospital of Lanzhou University, Lanzhou Gansu 730030, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2006 Jan;20(1):5-8.
To investigate the biocompatibility of diamond-like carbon (DLC) coated Nickel-Titanium shape memory alloy with osteoblasts cultured in vitro.
Rabbit's osteoblasts were incubated with DLC-coated Nickel-Titanium shape memory alloy disks and uncoated ones of equal size for 5 days. The control group (without shape memory alloy in culture media) was performed simultaneously. The cultured cells were counted and graphed. The samples from culture media were collected and the concentrations of alkaline phosphatase (ALP) and nickel (Ni2+) were measured from the 1st to 5th day respectively.
The proliferation of osteoblasts and the concentration of ALP in both DLC-coated group and control group was higher than uncoated group. The proliferation of osteoblasts on the 3rd, 4th, and 5th day in both DLC-coated group and control group was significantly higher than that in the uncoated group (P < 0.05). The concentration of ALP in DLC-coated group on the 2nd, 3rd, and 5th day and in the control group on the 3rd, 4th, and 5th day was significantly higher than that in the uncoated group (P < 0.05). The concentration of Ni2+ on the 3rd, 4th, and 5th day was significantly lower than that in the uncoated group (P < 0.05).
DLC- coated Nickel-Titanium shape memory alloys appears to have better biocompatibility with osteoblast cultured in vitro compared to uncoated ones.
研究类金刚石碳（DLC）涂层镍钛形状记忆合金与体外培养的成骨细胞的生物相容性。
将兔成骨细胞与DLC涂层镍钛形状记忆合金圆盘及同等大小的未涂层圆盘共同孵育5天。同时设立对照组（培养基中无形状记忆合金）。对培养的细胞进行计数并绘制图表。收集培养基样本，分别在第1天至第5天测量碱性磷酸酶（ALP）和镍（Ni2+）的浓度。
DLC涂层组和对照组中成骨细胞的增殖以及ALP的浓度均高于未涂层组。DLC涂层组和对照组在第3天、第4天和第5天的成骨细胞增殖明显高于未涂层组（P < 0.05）。DLC涂层组在第2天、第3天和第5天以及对照组在第3天、第4天和第5天的ALP浓度明显高于未涂层组（P < 0.05）。第3天、第4天和第5天的Ni2+浓度明显低于未涂层组（P < 0.05）。
与未涂层的镍钛形状记忆合金相比，DLC涂层的镍钛形状记忆合金与体外培养的成骨细胞似乎具有更好的生物相容性。
