Liu George E
USDA, ARS, ANRI, Bovine Functional Genomics Laboratory, Beltsville Agricultural Research Center (BARC) - East, Beltsville, MD 20705, USA.
Recent Pat Food Nutr Agric. 2009 Jan;1(1):75-9. doi: 10.2174/2212798410901010075.
The next-generation sequencing technologies are able to produce millions of short sequence reads in a high-throughput, cost-effective fashion. The emergence of these technologies has not only facilitated genome sequencing but also started to change the landscape of life sciences. Here, I survey their major applications ranging from whole-genome sequencing and resequencing, single nucleotide polymorphism (SNP) and structural variation discovery, to mRNA and noncoding RNA profiling and protein-nucleic acid interaction assay. These case studies in structural, functional and comparative genomics, metagenomics, and epigenomics are providing a more complete picture of the genome structures and functions. In the near future, we will witness broad impacts of these next-generation sequencing technologies for solving the complex biological problems in food, nutrition and agriculture. In this article, recent patents based information is also included.
新一代测序技术能够以高通量、高性价比的方式产生数百万条短序列读数。这些技术的出现不仅推动了基因组测序，还开始改变生命科学的格局。在这里，我概述了它们的主要应用，从全基因组测序和重测序、单核苷酸多态性（SNP）和结构变异发现，到mRNA和非编码RNA分析以及蛋白质-核酸相互作用测定。这些在结构、功能和比较基因组学、宏基因组学和表观基因组学方面的案例研究，正在提供关于基因组结构和功能的更完整图景。在不久的将来，我们将见证这些新一代测序技术对解决食品、营养和农业领域复杂生物学问题产生的广泛影响。本文还纳入了基于近期专利的信息。
