Liu George E
USDA, ARS, ANRI, Bovine Functional Genomics Laboratory, Beltsville Agricultural Research Center - East, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
Recent Pat Food Nutr Agric. 2011 Sep;3(3):187-95. doi: 10.2174/2212798411103030187.
Next-generation DNA sequencing technologies are able to produce millions of short sequence reads in a highthroughput, cost-effective fashion. The emergence of these technologies has not only facilitated genome sequencing but also changed the landscape of life sciences. This review surveys their recent applications in food, nutrition and agriculture ranging from whole-genome sequencing and resequencing, RNA-seq and ChIP-seq, structural, functional and comparative genomics to metagenomics and epigenetics. We already began to witness broad impacts of these DNA sequencing technologies for solving the complex biological problems in food, nutrition and agriculture. In this article, recent patent-based information is also included.
新一代DNA测序技术能够以高通量、高性价比的方式产生数百万条短序列读数。这些技术的出现不仅推动了基因组测序，还改变了生命科学的格局。本综述探讨了它们近期在食品、营养和农业领域的应用，涵盖全基因组测序与重测序、RNA测序和染色质免疫沉淀测序、结构基因组学、功能基因组学、比较基因组学，以及宏基因组学和表观遗传学。我们已经开始见证这些DNA测序技术对解决食品、营养和农业领域复杂生物学问题所产生的广泛影响。本文还纳入了近期基于专利的信息。
