Dyke R J, McBride H, George A J, Hamblin T J, Stevenson F K
Lymphoma Research Unit, Tenovus Research Laboratory, General Hospital, Southampton, United Kingdom.
Cell Immunol. 1991 Jan;132(1):70-83. doi: 10.1016/0008-8749(91)90007-x.
Idiotypic immunoglobulin, which can be considered to bear tumour-associated antigens in the context of B-cell lymphoma, has been obtained from the splenic A31 tumour, purified, and used to immunise syngeneic mice. On subsequent exposure to a lethal challenge of lymphoma cells, the mice showed no overt tumour development over an observation period of 6 months, whereas mice immunised with an unrelated idiotypic immunoglobulin succumbed to lymphoma after about 20 days. Anti-idiotypic immunity persisted in protected mice, since a second exposure to a lethal tumour dose 4 months after the first challenge also failed to induce lymphoma. Anti-idiotypic antibody appeared to have a major role in protection when analysed by passive transfer experiments, with no contribution from transferred cells. Protected mice were investigated for the presence of lymphoma cells 4-8 months following exposure to tumour, but the spleens, which were of normal weight and appearance, contained few or no tumour cells by phenotypic analysis. However, passage of cells dispersed from these spleens led, in 60% of cases, to tumour development in unimmunised recipients. The emergent tumours were indistinguishable from the original A31 lymphoma, with no evidence for variants, indicating that the cells were unable to grow in the immune mice, but that this dormant state could be disrupted by transfer.
独特型免疫球蛋白在B细胞淋巴瘤的背景下可被视为携带肿瘤相关抗原，已从脾脏A31肿瘤中获取，经纯化后用于免疫同基因小鼠。在随后接受淋巴瘤细胞的致死性攻击时，这些小鼠在6个月的观察期内未出现明显的肿瘤发展，而用无关独特型免疫球蛋白免疫的小鼠在约20天后死于淋巴瘤。抗独特型免疫在受保护的小鼠中持续存在，因为在首次攻击4个月后再次接受致死性肿瘤剂量攻击也未能诱发淋巴瘤。通过被动转移实验分析，抗独特型抗体在保护中似乎起主要作用，转移的细胞无贡献。在接触肿瘤4至8个月后，对受保护的小鼠进行淋巴瘤细胞存在情况的调查，但其脾脏重量和外观正常，通过表型分析发现几乎没有或没有肿瘤细胞。然而，从这些脾脏分散的细胞传代后，在60%的情况下会导致未免疫受体发生肿瘤。新出现的肿瘤与原始A31淋巴瘤无法区分，没有变体的证据，这表明细胞在免疫小鼠中无法生长，但这种休眠状态可因转移而被打破。
