Fang W G
Department of Pathology, Beijing Medical University.
Zhonghua Bing Li Xue Za Zhi. 1991 Mar;20(1):4-6.
In order to study the genetic mechanisms involved in tumor metastasis, high molecular weight DNA fragments were extracted from human PG tumor cells which were known to be highly metastatic in the nude mice. The DNA fragments were incorporated into NIH/3T3 cells by using calcium phosphate precipitate method. The transfected NIH/3T3 cells were tumorigenic and after a second-round transfection, one of the transfectants (TF87-2-10) was found metastatic to the lungs of nude mice. Human Alu sequences were detected in TF87-2-10 cells by Southern blot analysis. So, it seems that the metastatic capacity of TF87-2-10 cells is associated with the transfection of DNA fragments from PG tumor cells. The relationship between expression of metastatic phenotype of TF87-2-10 cells and host immune mechanisms was studied by injecting TF87-2-10 cells into athymic nude mice and beige nude mice. TF87-2-10 cells showed a higher metastatic rate in beige nude mice (6/13, 46%) than in the athymic nude mice (5/25, 20%). The results suggest that the metastatic expression of TF87-2-10 cells is subject to the influence of host NK cell activities.
为了研究肿瘤转移所涉及的遗传机制，从已知在裸鼠中具有高转移能力的人PG肿瘤细胞中提取了高分子量DNA片段。通过磷酸钙沉淀法将DNA片段导入NIH/3T3细胞。转染后的NIH/3T3细胞具有致瘤性，在第二轮转染后，发现其中一个转染子（TF87-2-10）转移至裸鼠肺部。通过Southern印迹分析在TF87-2-10细胞中检测到了人Alu序列。因此，TF87-2-10细胞的转移能力似乎与PG肿瘤细胞DNA片段的转染有关。通过将TF87-2-10细胞注射到无胸腺裸鼠和米色裸鼠中，研究了TF87-2-10细胞转移表型的表达与宿主免疫机制之间的关系。TF87-2-10细胞在米色裸鼠中的转移率（6/13，46%）高于无胸腺裸鼠（5/25，20%）。结果表明，TF87-2-10细胞的转移表达受宿主NK细胞活性的影响。
