富血小板血浆应用于跟腱节段模型后 TGF-β1 的时空表达。
Temporal and spatial expression of TGF-beta1 in an Achilles tendon section model after application of platelet-rich plasma.
机构信息
Democritus University of Thrace, Department of Orthopaedic and Trauma Surgery, Dragana, BO 84100, Greece.
出版信息
Foot Ankle Surg. 2010 Sep;16(3):137-41. doi: 10.1016/j.fas.2009.09.002. Epub 2009 Nov 12.
BACKGROUND
To investigate the effect of platelet-rich plasma (PRP) on TGF-beta1 expression during tendon healing.
METHODS
We used 48 skeletally mature New Zealand White rabbits. 24 rabbits received the PRP, and 24 rabbits served as an untreated control group. Equal numbers of animals were sacrificed at 1st, 2nd, 3rd, and 4th week. The surgical procedure involved a transverse incision to transect the Achilles tendon. A volume of 1ml of PRP was then injected into the tendon mass in the PRP group. Histological and immunohistochemical evaluations with an anti-TGF-beta primary antibody were performed.
RESULTS
The pattern of expression of TGF-beta1 in the PRP group was characterized by a significant upregulation during the first 2 weeks and subsequently significant downregulation in the 3rd and 4th week in comparison with the controls.
CONCLUSIONS
Our results suggest that PRP may affect the tendon healing process by altering the expression of TGF-beta1.
背景
研究富血小板血浆（PRP）对肌腱愈合过程中 TGF-β1 表达的影响。
方法
我们使用 48 只骨骼成熟的新西兰白兔。24 只兔子接受了 PRP 治疗，24 只兔子作为未治疗的对照组。第 1、2、3 和 4 周分别处死等量的动物。手术过程包括横切口横断跟腱。在 PRP 组中将 1ml 的 PRP 注入肌腱块中。用抗 TGF-β1 一抗进行组织学和免疫组织化学评价。
结果
与对照组相比，PRP 组 TGF-β1 的表达模式在最初 2 周内显著上调，随后在第 3 和 4 周内显著下调。
结论
我们的结果表明，PRP 可能通过改变 TGF-β1 的表达来影响肌腱愈合过程。
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