Department of Laboratory Medicine, the First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.
Int J Infect Dis. 2010 Sep;14(9):e815-22. doi: 10.1016/j.ijid.2010.04.005. Epub 2010 Jul 22.
Immune regulatory mechanisms may limit the immunopathologic condition of infection with Mycobacterium tuberculosis and suppress cellular immune responses in the host. We investigated the CD4(+)CD25(+)FoxP3(+) circulating regulatory T cells (T(reg)) in patients with cavity multidrug-resistant tuberculosis (MDR-TB) before and after surgery.
We compared the proportion of T(reg) cells in 13 patients with cavity MDR-TB pre- and postoperatively and in 10 healthy control subjects by flow cytometry using three specific markers in peripheral blood lymphocytes: cell-surface CD4 and CD25 expression and intracellular FoxP3 expression.
The proportion of CD4(+)CD25(high) and CD4(+)CD25(+)FoxP3(+) T(reg) was significantly higher in patients with cavity MDR-TB and at 1-month postoperatively than in healthy controls (p<0.001). The proportion of CD4(+) and CD4(+)CD25(-) cells was significantly lower in patients with cavity MDR-TB than in controls (p<0.001). Pre- and postoperative proportions of CD4(+)CD25(high) and CD4(+)CD25(+)FoxP3(+) T(reg) cells showed a positive correlation (r=0.878, p<0.001).
Circulating T(reg) cells are increased in proportion in patients with cavity MDR-TB and decreased after surgery. Infection with M. tuberculosis may induce T(reg) cell-surface molecular changes with increased numbers of cells.
免疫调节机制可能会限制结核分枝杆菌感染的免疫病理状况，并抑制宿主的细胞免疫反应。我们研究了手术前后空洞型耐多药肺结核（MDR-TB）患者外周血中 CD4+CD25+FoxP3+调节性 T 细胞（Treg）的比例。
我们通过流式细胞术，使用外周血淋巴细胞上的三个特异性标记物（细胞表面 CD4 和 CD25 的表达以及细胞内 FoxP3 的表达），比较了 13 例空洞型 MDR-TB 患者术前和术后以及 10 例健康对照者外周血中 Treg 细胞的比例。
空洞型 MDR-TB 患者和术后 1 个月患者的 CD4+CD25（高）和 CD4+CD25+FoxP3+Treg 的比例明显高于健康对照组（p<0.001）。空洞型 MDR-TB 患者的 CD4+和 CD4+CD25（-）细胞的比例明显低于对照组（p<0.001）。术前和术后 CD4+CD25（高）和 CD4+CD25+FoxP3+Treg 细胞的比例呈正相关（r=0.878，p<0.001）。
空洞型 MDR-TB 患者外周血中 Treg 细胞的比例增加，术后减少。结核分枝杆菌感染可能会诱导 Treg 细胞表面分子的改变，导致细胞数量增加。
