Key Laboratory of Otolaryngology-Head and Neck Surgery, Capital Medical University, Ministry of Education of China, Beijing Institute of Otorhinolaryngology, Beijing 100005, China.
Head Neck. 2011 Jul;33(7):1005-12. doi: 10.1002/hed.21581. Epub 2010 Dec 6.
The purpose of this study was to investigate the presence of CD4(+) CD25(+) FOXP3(+) regulatory T (Treg) cells both in peripheral blood and local tumors in patients with nasal inverted papilloma (NIP).
By using flow cytometry, the frequencies of CD4(+) CD25(+) FOXP3(+) Treg cells in both peripheral blood and tissues from 18 patients with NIP and 8 control subjects were determined. CCL22 and CCL17 proteins in NIP tumors were analyzed by using enzyme-linked immunosorbent assay. The suppressive capacity of Treg cells was estimated by WST-8 and enzyme-linked immunosorbent assay (ELISA; interferon-gamma [IFN-γ] and interleukin-4 [IL-4]) analysis.
Patients with NIP showed increased CD4(+) CD25(+) FOXP3(+) Treg cell frequencies in tumor tissues and CD4(+) T cell fraction rather than in peripheral blood. CCL22 increased in NIP tumors. Phytohemagglutinin (PHA)-induced proliferation and cytokine production of CD4(+) CD25(-) T cells were suppressed equally well by CD4(+) CD25(high) cells from both patients with NIP and controls.
Our study demonstrated the increased frequencies of CD4(+) CD25(+) FOXP3(+) Treg cells in NIP tumors, which might be influenced by CCL22.
本研究旨在探讨鼻内翻性乳头状瘤（NIP）患者外周血和局部肿瘤中 CD4(+) CD25(+) FOXP3(+) 调节性 T（Treg）细胞的存在情况。
采用流式细胞术检测 18 例 NIP 患者和 8 例对照者外周血和组织中 CD4(+) CD25(+) FOXP3(+) Treg 细胞的频率。采用酶联免疫吸附试验（ELISA）分析 NIP 肿瘤中 CCL22 和 CCL17 蛋白。采用 WST-8 和 ELISA（干扰素-γ[IFN-γ]和白细胞介素-4[IL-4]）分析 Treg 细胞的抑制能力。
NIP 患者肿瘤组织和 CD4(+) T 细胞亚群中 CD4(+) CD25(+) FOXP3(+) Treg 细胞频率增加，而外周血中则未增加。NIP 肿瘤中 CCL22 增加。植物血凝素（PHA）诱导的 CD4(+) CD25(-) T 细胞增殖和细胞因子产生均可被 NIP 患者和对照者的 CD4(+) CD25(high)细胞同等程度地抑制。
本研究表明，NIP 肿瘤中 CD4(+) CD25(+) FOXP3(+) Treg 细胞频率增加，可能受 CCL22 影响。
