Zheng S S, Wai W L, Hin W Y, Wu A R
Department of Immunology, Chinese Academy of Medical Sciences, WHO Collaborating Centre for Immunological Research, Beijing.
Chin Med J (Engl). 1991 Apr;104(4):292-9.
Allergenicity of three plant abortifacient proteins, trichosanthin (TCS), alpha-momorcharin (alpha-MMC) and beta-momorcharin (beta-MMC) and the kinetics of IgE antibody response against these proteins were studied in C57BL/6N and BALB/cAn mice. These proteins were purified from Chinese medicinal herbs, characterized by biochemical and immunological procedures and found to be homogeneous by PAGE, SDS-PAGE and immunoelectrophoresis. The results obtained were summarized as follows: 1) different levels of IgE antibody responses against TCS, alpha-MMC and beta-MMC were induced when they were injected i.p. as antigen adsorbed onto AI(OH)3 gel into BALB/cAn or C57BL/6N mice on day 0 and day 28; 2) the allergenicity of the proteins was in the order of beta-MMC greater than alpha-MMC greater than TCS; 3) two injections of 10 micrograms or 50 micrograms of TCS or alpha-MMC given at 4 weeks interval without adjuvant elicited a low or undetectable PCA titer, while the same dose given weekly for 5 weeks resulted in high levels of IgE production; and 4) on the basis of PCA observations, no cross immunological reactivity among these three proteins was found, it seemed to support that TCS, alpha-MMC and beta-MMC may be alternately used for inducing abortion.
在C57BL/6N和BALB/cAn小鼠中研究了三种植物堕胎蛋白天花粉蛋白（TCS）、α-苦瓜素（α-MMC）和β-苦瓜素（β-MMC）的致敏性以及针对这些蛋白的IgE抗体反应动力学。这些蛋白从中药材中纯化得到，通过生化和免疫学方法进行表征，并通过PAGE、SDS-PAGE和免疫电泳证明其均一性。所得结果总结如下：1）当在第0天和第28天将吸附在氢氧化铝凝胶上作为抗原的TCS、α-MMC和β-MMC腹腔注射到BALB/cAn或C57BL/6N小鼠体内时，诱导出了不同水平的针对它们的IgE抗体反应；2）这些蛋白的致敏性顺序为β-MMC大于α-MMC大于TCS；3）间隔4周无佐剂注射两次10微克或50微克的TCS或α-MMC引发的PCA滴度较低或无法检测到，而每周注射相同剂量持续5周则导致高水平的IgE产生；4）基于PCA观察，未发现这三种蛋白之间存在交叉免疫反应性，这似乎支持TCS、α-MMC和β-MMC可交替用于引产。
