National Center for Antimicrobials & Infection Control, Statens Serum Institut, Copenhagen S, Denmark.
Int J Food Microbiol. 2010 Aug 15;142(1-2):264-72. doi: 10.1016/j.ijfoodmicro.2010.06.025. Epub 2010 Jul 3.
Urinary tract infection (UTI) is one of the most common bacterial infections. UTI is primarily caused by extraintestinal pathogenic Escherichia coli (ExPEC) from the patients' own fecal flora. The ExPEC often belong to phylogroups B2 and D, the groups which include potent human ExPEC isolates causing UTI, bacteremia, and meningitis. The external sources of these ExPEC in the human intestine are unknown. The food supply may transmit ExPEC to humans. However, evidence of this hypothesis is limited. To assess this hypothesis, the objective of our study was to investigate the presence of ExPEC related virulence genes in E. coli isolates from UTI patients, community-dwelling humans, meat, and production animals. Accordingly, we included 964 geographically and temporally matched E. coli isolates from UTI patients (n=102), community-dwelling humans (n=109), fresh Danish (n=197) and imported broiler chicken meat (n=86), broiler chickens (n=138), fresh Danish (n=177) and imported pork (n=10), and pigs (n=145) in the study. All isolates were investigated for the presence of eight ExPEC related genes (kpsM II, papA, papC, iutA, sfaS, focG, afa, hlyD) using PCR. To investigate any similarities between isolates from the different origins, we performed a cluster analysis including antimicrobial resistance data previously published. We detected seven of the eight ExPEC related genes in isolates from broiler chicken meat, broiler chickens, pork and pigs. Our findings suggest that broiler chicken meat, broiler chickens, pork and pigs could be the source of strains with these ExPEC related virulence genes in community-dwelling humans and UTI patients. Especially detection of ExPEC related virulence genes in isolates belonging to phylogroups B2 and D is very concerning and may have a significant medical impact. The cluster analysis of virulence gene and antimicrobial resistance profiles showed strong similarities between UTI patient, community-dwelling human isolates, meat, and production animal isolates. Thus, these strains from meat and production animals may pose a zoonotic risk.
尿路感染（UTI）是最常见的细菌性感染之一。UTI 主要由患者自身粪便菌群中的肠外致病性大肠杆菌（ExPEC）引起。ExPEC 通常属于 B2 和 D 群，这些群包括引起 UTI、菌血症和脑膜炎的强人类 ExPEC 分离株。人类肠道中这些 ExPEC 的外部来源尚不清楚。食物供应可能会将 ExPEC 传播给人类。然而，这一假设的证据有限。为了评估这一假设，我们的研究目的是调查 UTI 患者、社区居民、肉类和养殖动物中分离的大肠杆菌中与 ExPEC 相关的毒力基因的存在情况。因此，我们纳入了 964 株在地理位置和时间上与 UTI 患者（n=102）、社区居民（n=109）、丹麦新鲜（n=197）和进口肉鸡（n=86）、肉鸡（n=138）、丹麦新鲜（n=177）和进口猪肉（n=10）以及猪（n=145）相匹配的大肠杆菌分离株，使用 PCR 方法检测这些分离株中存在的 8 种与 ExPEC 相关的基因（kpsM II、papA、papC、iutA、sfaS、focG、afa 和 hlyD）。为了研究不同来源的分离株之间是否存在相似性，我们进行了聚类分析，包括之前发表的抗菌药物耐药性数据。我们在来自肉鸡、肉鸡、猪肉和猪的分离株中检测到了 8 种与 ExPEC 相关的基因中的 7 种。我们的研究结果表明，肉鸡、肉鸡、猪肉和猪可能是社区居民和 UTI 患者中携带这些与 ExPEC 相关毒力基因的菌株的来源。特别是在属于 B2 和 D 群的分离株中检测到与 ExPEC 相关的毒力基因是非常令人担忧的，可能具有重要的医学影响。毒力基因和抗菌药物耐药性谱的聚类分析显示，UTI 患者、社区居民分离株、肉类和养殖动物分离株之间存在很强的相似性。因此，这些来自肉类和养殖动物的菌株可能存在动物源性病原体感染的风险。
