Zhou Qian, Tang Mengjun, Zhang Xiaoyan, Tang Xiujun, Lu Junxian, Gao Yushi
Jiangsu Institute of Poultry Science, Yangzhou, China.
Front Vet Sci. 2024 Nov 28;11:1500355. doi: 10.3389/fvets.2024.1500355. eCollection 2024.
The prevalence of antimicrobial resistance originating from animals presents a significant threat to the treatment of animal disease, public health, and food safety. Researchers have focused on antibiotic resistance in (), yet there are few reports on the resistance change during the feeding cycle. The purpose of this study was to investigate the prevalence and antibiotic resistance changes of in animal, environmental, and human samples during the broiler feeding cycle. Epidemiological surveys were performed in a farm with feeding AA broilers in Yangzhou, Jiangsu Province, China. Results showed that during a 42-days feeding cycle, 128 isolates were obtained from the cloaca of white-feathered broilers ( = 140), with an isolation rate of 91.4%, 27 isolates were obtained from Feed ( = 70) and 35 isolates were obtained from cage swabs ( = 70). A workers' hands swabs sample isolation rate of 68.6% (24/35) was observed. Antibiotic susceptibility testing revealed that out of 214 isolates, varying degrees of resistance were observed against 14 antibiotics. Most strains were resistant to ampicillin, cephalothiophene, ciprofloxacin, tetracycline, sulfamisoxazole, sulfamethoxazole and florfenicol, with a resistance rate exceeding 80%. The resistant strains demonstrated relatively stable patterns in their resistance to various antibiotics. Of the six antibiotic resistance genes tested, the gene showed the highest detection rate (72.4%), followed by (43.0%), (35.0%), (28.0%), (8.4%), (3.7%), and (0). The highest detection rate for virulence genes was . In summary, the isolation rate of and antibiotic resistance profile in broiler chickens remained stable throughout their feeding cycle. These findings can serve as a reference for the rational use of antibiotics in clinical settings, they can guide the use of veterinary drugs in poultry breeding.
源自动物的抗菌药物耐药性对动物疾病治疗、公共卫生和食品安全构成重大威胁。研究人员一直关注（）中的抗生素耐药性，但关于饲养周期内耐药性变化的报道较少。本研究的目的是调查肉鸡饲养周期内动物、环境和人类样本中（）的流行情况及抗生素耐药性变化。在中国江苏省扬州市一个饲养AA肉鸡的农场进行了流行病学调查。结果显示，在42天的饲养周期内，从白羽肉鸡泄殖腔（n = 140）中分离出128株（），分离率为91.4%，从饲料（n = 70）中分离出27株，从笼具拭子（n = 70）中分离出35株。观察到工人手部拭子样本分离率为68.6%（24/35）。抗生素敏感性测试显示，在214株（）分离株中，观察到对14种抗生素有不同程度的耐药性。大多数菌株对氨苄西林、头孢噻吩、环丙沙星、四环素、磺胺异恶唑、磺胺甲恶唑和氟苯尼考耐药，耐药率超过80%。耐药菌株对各种抗生素的耐药模式相对稳定。在所检测的6种抗生素耐药基因中，（）基因检测率最高（72.4%），其次是（）（43.0%）、（）（35.0%）、（）（28.0%）、（）（8.4%）、（）（3.7%）和（）（0）。毒力基因的最高检测率是（）。总之，肉鸡在整个饲养周期内（）的分离率和抗生素耐药谱保持稳定。这些发现可为临床合理使用抗生素提供参考，可指导家禽养殖中兽药的使用。
