Burrows A L, Hill H A, Leese T A, Mcintire W S, Nakayama H, Sanghera G S
Inorganic Chemical Laboratory, University of Oxford, England.
Eur J Biochem. 1991 Jul 1;199(1):73-8. doi: 10.1111/j.1432-1033.1991.tb16093.x.
The electrochemical response of methylamine dehydrogenase from bacterium W3A1 at edge-plane-oriented pyrolytic graphite (epg) and modified gold electrodes has been investigated. Quasi-reversible electron transfer has been observed. Variations in concentration of different cations and anions gave rise to both promotion and inhibition of the direct response. A catalytic response of the enzyme in the presence of methylamine has been observed at both an epg electrode and a 2,2'-dithiodiglycolic-acid-modified gold electrode surface, and the effects of various cations and anions on the catalytic peak current have been investigated. The spectroelectrochemical results obtained at an optically transparent thin-layer electrode, modified with 2,2'-dithiodiglycolic acid, are also reported. In the presence of 1,1'-dimethylferrocene-3-(1-ethanol-2-amine) (14.8 microM), the results reveal a midpoint potential of -148 mV for methylamine dehydrogenase from bacterium W3A1. This is in very close agreement to the value obtained in the cyclic voltammetric investigations of -140 mV.
对来自细菌W3A1的甲胺脱氢酶在边缘平面取向热解石墨（epg）电极和修饰金电极上的电化学响应进行了研究。观察到了准可逆电子转移。不同阳离子和阴离子浓度的变化导致直接响应既有促进作用也有抑制作用。在epg电极和2,2'-二硫代二乙醇酸修饰的金电极表面均观察到该酶在甲胺存在下的催化响应，并研究了各种阳离子和阴离子对催化峰电流的影响。还报道了在经2,2'-二硫代二乙醇酸修饰的光学透明薄层电极上获得的光谱电化学结果。在存在1,1'-二甲基二茂铁-3-(1-乙醇-2-胺)（14.8 microM）的情况下，结果显示来自细菌W3A1的甲胺脱氢酶的中点电位为-148 mV。这与循环伏安法研究中获得的-140 mV的值非常接近。
