Department of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Sagamihara, Kanagawa 228-8555, Japan.
Cancer Genomics Proteomics. 2010 Jul-Aug;7(4):181-9.
To effectively obtain tumour-specific markers, fractionated proteins obtained using reversed-phase high-performance liquid chromatography for patient-matched pre- and postoperative sera from bladder cancer patients were compared by two-dimensional gel electrophoresis. The usefulness of the identified proteins was confirmed immunohistochemically. S100A8 and S100A9 were identified as tumour-associated proteins. The increased immunoreactive expression of S100A8 protein was associated with bladder wall muscle invasion of the tumour and cancer-specific survival (p<0.05), and the increased immunoreactive expression of S100A9 protein was associated with the tumour grade (p<0.05). In addition, increased expressions of both proteins was associated with recurrence-free survival at a median follow-up of 32.9 months (both p<0.05). On multivariate analysis, the expression of S100A8 was a significant predictor of recurrence (p<0.05). These findings may help to identify biologically aggressive tumors and, thus, patients who might benefit from more intensive adjuvant therapy.
为了有效获得肿瘤特异性标志物，通过二维凝胶电泳比较了膀胱癌患者配对术前和术后血清中反相高效液相色谱法分离的分段蛋白质。通过免疫组织化学法证实了所鉴定蛋白质的有用性。S100A8 和 S100A9 被鉴定为肿瘤相关蛋白。S100A8 蛋白免疫反应性表达的增加与肿瘤壁肌肉侵犯和癌症特异性生存相关（p<0.05），S100A9 蛋白免疫反应性表达的增加与肿瘤分级相关（p<0.05）。此外，在中位随访 32.9 个月时，两种蛋白质的表达增加均与无复发生存相关（均 p<0.05）。多变量分析显示，S100A8 的表达是复发的显著预测因子（p<0.05）。这些发现可能有助于识别具有侵袭性生物学行为的肿瘤，从而使那些可能受益于更强化辅助治疗的患者受益。
