Wada E, Koyama-Ito H, Matsuzawa A
Department of Cell Chemistry, University of Tokyo, Japan.
Exp Eye Res. 1991 May;52(5):501-6. doi: 10.1016/0014-4835(91)90049-k.
Congenic hereditary cataract mice, BALB/c-nct/nct, were established by introducing the nct gene from Nakano into BALB/c mice. These mice developed a milder cortical form of cataract which developed sporadically and later in life than in Nakano mice. Combined use of BALB/c and BALB/c-nct/nct mice enables biochemical comparison of normal clear lenses, congenic clear lenses which are destined to be opacified some time later, and opacified lenses in the same genetic and aging statuses. We compared the age-related changes in water content and water-soluble and -insoluble fractions among these three types of lenses. Congenic clear lenses and opaque lenses were more similar to BALB/c normal clear lenses and Nakano opaque ones, respectively, in these parameters. These results suggest, in addition to formation of aggregated crystallins and their accumulation in water-insoluble fractions, that decreased protein synthesis, increased protein degradation and augmented leakage of crystallin might have a significant role in the nct-induced lens opacification.
通过将来自中野小鼠的nct基因导入BALB/c小鼠，建立了同基因遗传性白内障小鼠BALB/c-nct/nct。这些小鼠患有一种较温和的皮质性白内障，与中野小鼠相比，其发病较为散在且较晚。联合使用BALB/c小鼠和BALB/c-nct/nct小鼠，可以对正常透明晶状体、注定在一段时间后变混浊的同基因透明晶状体以及处于相同遗传和衰老状态的混浊晶状体进行生化比较。我们比较了这三种类型晶状体中与年龄相关的含水量、水溶性和水不溶性组分的变化。在这些参数方面，同基因透明晶状体和不透明晶状体分别与BALB/c正常透明晶状体和中野不透明晶状体更为相似。这些结果表明，除了晶状体蛋白聚集物的形成及其在水不溶性组分中的积累外，蛋白质合成减少、蛋白质降解增加以及晶状体蛋白渗漏增加可能在nct诱导的晶状体混浊中起重要作用。
