Matsuzawa A, Wada E
Laboratory Animal Research Center, University of Tokyo, Japan.
Exp Eye Res. 1988 Nov;47(5):705-11. doi: 10.1016/0014-4835(88)90038-3.
The Nakano cataract gene, nct, was introduced into Balb/c mice by repeated backcrosses to elucidate the possible effects of background genes on its expression. The resulting congenic Balb/c-nct/nct mice were characterized by retarded and sporadic cataract formation with a tendency of further retardation in males and by the different disease process of cataract as compared with Nakano mice. The age of 50% cataract incidence was 60 days in females and 90 days in males compared with 22 days in Nakano mice, and lens opacification commenced in a diffuse, mild form at the cortex in congenic but in a pin-head, intense form at the core in Nakano mice. Sex hormones seemed to be involved in the difference in cateractogenesis between male and female mice. Microphthalmia was slighter in degree in Balb/c background mice. The results indicated that the nct-dependent cataractogenesis may be influenced by background genes and some non-hereditary factors. Balb/c-nct/nct mice will provide a new type of hereditary cataract model.
通过反复回交将中野白内障基因nct导入Balb/c小鼠，以阐明背景基因对其表达可能产生的影响。由此产生的同源Balb/c-nct/nct小鼠的特征是白内障形成延迟且呈散发性，雄性有进一步延迟的趋势，并且与中野小鼠相比，白内障的疾病进程有所不同。50%白内障发病率的年龄在雌性中为60天，在雄性中为90天，而中野小鼠为22天，并且在同源小鼠中晶状体混浊以弥漫性、轻度形式在皮质开始，而在中野小鼠中以针尖状、强烈形式在核心开始。性激素似乎与雌雄小鼠白内障发生的差异有关。在Balb/c背景小鼠中小眼症程度较轻。结果表明，依赖nct的白内障发生可能受背景基因和一些非遗传因素的影响。Balb/c-nct/nct小鼠将提供一种新型的遗传性白内障模型。
