不同极性的武夷菌素组分的抗菌活性研究。

Antimicrobial activity investigation on Wuyiencin fractions of different polarity.

机构信息

State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

出版信息

Molecules. 2010 Apr 29;15(5):3179-85. doi: 10.3390/molecules15053179.

DOI:10.3390/molecules15053179

PMID:20657470

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6263320/

Abstract

The aim of this study was to evaluate the antimicrobial activity of Wuyiencin fractions with different polarities against six indicator microorganisms: Rhodotorula rubra, Bacillus subtilis, Bacillus megaterium, Escherichia coli, Cladosporium fulvum and Staphylococcus aureus. The fermentation broth of Wuyiencin was submitted to AB-8 macroporous adsorptive resin and fractionated with solvents of different polarity. The fraction eluted with water had remarkably antimicrobial activity against all the microorganisms investigated except for C. fulvum and S. aureus (MIC <or= 0.0625 mg/mL), probably due to the presence of active components. The fraction eluted with methanol showed potential antimicrobial activity against all the test microorganisms except for R.rubra, with MIC values of 0.5 and 2 mg/mL. In conclusion, fractions eluted with water and methanol, respectively, represent the main active-part of Wuyiencin, and could be emphasized for agricultural applications in the future.

摘要

本研究旨在评估不同极性的武夷菌素级分对六种指示微生物的抗菌活性

红酵母、枯草芽孢杆菌、巨大芽孢杆菌、大肠杆菌、藤仓镰刀菌和金黄色葡萄球菌。武夷菌素发酵液经 AB-8 大孔吸附树脂处理，用不同极性的溶剂进行分级。用水洗脱的级分对除藤仓镰刀菌和金黄色葡萄球菌以外的所有研究微生物均具有显著的抗菌活性（MIC <= 0.0625 mg/mL），这可能是由于存在活性成分。用甲醇洗脱的级分对除红酵母以外的所有测试微生物均具有潜在的抗菌活性，MIC 值为 0.5 和 2 mg/mL。综上所述，分别用水和甲醇洗脱的级分代表了武夷菌素的主要活性部分，未来可能会在农业应用中得到重视。