Department of Chemistry, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia.
Molecules. 2010 May 7;15(5):3378-90. doi: 10.3390/molecules15053378.
The in vitro and in vivo antioxidant activities of different extracts of laurel leaves were studied. Free radical scavenging capacity (RSC) was evaluated measuring the scavenging activity on the DPPH, NO, O(2)(.-) and OH radicals. The effects on lipid peroxidation (LP) were also evaluated. Experimental results indicate that ethyl acetate extract of leaves has exhibited the largest RSC capacity in neutralization of DPPH, NO, O(2)(.-) and OH radicals. The same result was obtained in investigation of extracts impact on LP. The in vivo effects were evaluated on some antioxidant systems (activities of GSHPx, LPx, Px, CAT and XOD, and GSH content) in the mice liver and blood-hemolysate after treatment with the examined laurel extracts, or in combination with carbon tetrachloride (CCl(4)). On the basis of the results obtained it can be concluded that the examined extracts exhibited a certain protective effect, which is more pronounced on the liver than on blood-hemolysate parameters. The results obtained indicate toxicity of CCl(4), probably due to the radicals involved in its metabolism. Combined treatments with CCl(4) and the examined extracts showed both positive and negative synergism. Based on the experimental results, the strongest protective effect was shown by the EtOAc extract.
研究了月桂叶不同提取物的体外和体内抗氧化活性。通过测量对 DPPH、NO、O(2)(.-) 和 OH 自由基的清除活性来评估自由基清除能力 (RSC)。还评估了对脂质过氧化 (LP) 的影响。实验结果表明，叶的乙酸乙酯提取物在中和 DPPH、NO、O(2)(.-) 和 OH 自由基方面表现出最大的 RSC 能力。在研究提取物对 LP 的影响时也得到了相同的结果。体内效应在小鼠肝脏和血液溶血后用检查的月桂叶提取物处理后，或与四氯化碳 (CCl(4)) 联合处理后，评估了一些抗氧化系统（GSHPx、LPx、Px、CAT 和 XOD 的活性以及 GSH 含量）。基于所获得的结果，可以得出结论，所检查的提取物表现出一定的保护作用，对肝脏的保护作用比血液溶血参数更明显。结果表明 CCl(4)具有毒性，可能是由于其代谢过程中涉及自由基。用 CCl(4)和检查的提取物联合处理显示出正协同和负协同作用。基于实验结果，乙酸乙酯提取物表现出最强的保护作用。
