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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Silva S V, Mercer J R

Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.

Int J Biochem. 1991;23(5-6):525-9. doi: 10.1016/0020-711x(87)90044-9.

Isolated cat liver cells were established in monolayer culture and intracellular proteins were labelled for 20 hr with L-[4,5-3H]leucine. 2. The known intralysosomal protein degradation inhibitors such as NH4Cl, leupeptin, cycloheximide and insulin were all effective in decreasing the rate of proteolysis in cat hepatocytes. However, the maximum inhibitory effects of these agents were generally lower when compared to the rat. 3. These results indicated a smaller contribution of the lysosomal pathway towards overall protein degradation in cat liver.

将分离的猫肝细胞进行单层培养，并用L-[4,5-³H]亮氨酸对细胞内蛋白质进行20小时的标记。2. 已知的溶酶体内蛋白质降解抑制剂，如氯化铵、亮抑酶肽、环己酰亚胺和胰岛素，均能有效降低猫肝细胞中的蛋白水解速率。然而，与大鼠相比，这些药物的最大抑制作用通常较低。3. 这些结果表明，溶酶体途径对猫肝脏整体蛋白质降解的贡献较小。

Silva S V, Mercer J R

Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.

Int J Biochem. 1991;23(5-6):525-9. doi: 10.1016/0020-711x(87)90044-9.

Isolated cat liver cells were established in monolayer culture and intracellular proteins were labelled for 20 hr with L-[4,5-3H]leucine. 2. The known intralysosomal protein degradation inhibitors such as NH4Cl, leupeptin, cycloheximide and insulin were all effective in decreasing the rate of proteolysis in cat hepatocytes. However, the maximum inhibitory effects of these agents were generally lower when compared to the rat. 3. These results indicated a smaller contribution of the lysosomal pathway towards overall protein degradation in cat liver.

将分离的猫肝细胞进行单层培养，并用L-[4,5-³H]亮氨酸对细胞内蛋白质进行20小时的标记。2. 已知的溶酶体内蛋白质降解抑制剂，如氯化铵、亮抑酶肽、环己酰亚胺和胰岛素，均能有效降低猫肝细胞中的蛋白水解速率。然而，与大鼠相比，这些药物的最大抑制作用通常较低。3. 这些结果表明，溶酶体途径对猫肝脏整体蛋白质降解的贡献较小。