正常成纤维细胞接近汇合时蛋白水解的调控。溶酶体系统参与的证据。

The regulation of proteolysis in normal fibroblasts as they approach confluence. Evidence for the participation of the lysosomal system.

作者信息

Cockle S M, Dean R T

出版信息

Biochem J. 1982 Dec 15;208(3):795-800. doi: 10.1042/bj2080795.

DOI:10.1042/bj2080795

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1154033/

Abstract

The effect of the lysosomotropic agent NH4Cl and the proteinase inhibitors leupeptin, Z-Phe-Ala-CHN2 (benzyloxycarbonylphenylalanylalanyldiazomethane) and pepstatin on the degradation of intracellular proteins in Swiss 3T3 mouse and normal human fibroblasts in both the exponential and stationary (confluent) growth phases in nutritionally complete conditions was investigated. Inhibitory effects of all four agents on degradation in both growth states were detected. The increase in proteolysis normally occurring as cells approach confluence could be completely blocked by NH4Cl, by Z-Phe-Ala-CHN2, or by pepstatin in the presence of leupeptin. These results suggest that the lysosomal system is responsible for the regulation of proteolysis at confluence and further confirm its role in 'basal' proteolysis in growing cells.

摘要

研究了溶酶体促渗剂氯化铵以及蛋白酶抑制剂亮抑酶肽、Z-苯丙氨酰丙氨酸重氮甲烷和胃蛋白酶抑制剂对营养完全条件下处于指数生长期和平静期（汇合期）的瑞士3T3小鼠及正常人成纤维细胞内蛋白质降解的影响。检测到这四种试剂对两种生长状态下的蛋白质降解均有抑制作用。当细胞接近汇合时正常发生的蛋白水解增加可被氯化铵、Z-苯丙氨酰丙氨酸重氮甲烷或在亮抑酶肽存在时被胃蛋白酶抑制剂完全阻断。这些结果表明溶酶体系统负责汇合时蛋白水解的调节，并进一步证实其在生长细胞“基础”蛋白水解中的作用。

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