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1
The regulation of proteolysis in normal fibroblasts as they approach confluence. Evidence for the participation of the lysosomal system.正常成纤维细胞接近汇合时蛋白水解的调控。溶酶体系统参与的证据。
Biochem J. 1982 Dec 15;208(3):795-800. doi: 10.1042/bj2080795.
2
An abnormality in intracellular protein degradation in fibroblasts from patients with I-cell disease.
Clin Chim Acta. 1984 Jul 31;140(3):257-65. doi: 10.1016/0009-8981(84)90207-9.
3
The inhibition of macrophage protein turnover by a selective inhibitor of thiol proteinases.巯基蛋白酶选择性抑制剂对巨噬细胞蛋白质周转的抑制作用。
Biochem J. 1980 Feb 15;186(2):385-90. doi: 10.1042/bj1860385.
4
Lysosomes and protein degradation.溶酶体与蛋白质降解
Ciba Found Symp. 1979(75):139-49. doi: 10.1002/9780470720585.ch9.
5
Biogenesis of lysosomes in marshall cells and in cells of the male reproductive system.马歇尔细胞及雄性生殖系统细胞中溶酶体的生物发生
Mol Reprod Dev. 2001 May;59(1):54-66. doi: 10.1002/mrd.1007.
6
[Contribution of lysosomal enzymes into degradation of short- and long-lived proteins in human embryonal fibroblasts with abnormal chromosome complement].[溶酶体酶对染色体组异常的人胚成纤维细胞中短寿命和长寿命蛋白质降解的贡献]
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7
The effect of lysosomal inhibitors on protein degradation in cat hepatocyte monolayers.溶酶体抑制剂对猫肝细胞单层中蛋白质降解的影响。
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8
The proteinase inhibitors leupeptin, pepstatin A, and TLCK cause reduced collagen production in freshly isolated embryonic chick fibroblasts in suspension culture.蛋白酶抑制剂亮抑酶肽、胃蛋白酶抑制剂A和甲苯磺酰-L-赖氨酸氯甲基酮可使悬浮培养的新鲜分离的胚胎鸡成纤维细胞中的胶原蛋白生成减少。
Arch Biochem Biophys. 1984 Sep;233(2):338-44. doi: 10.1016/0003-9861(84)90454-5.
9
In vitro embryotoxicity of the cysteine proteinase inhibitors benzyloxycarbonyl-phenylalanine-alanine-diazomethane (Z-Phe-Ala-CHN2) and benzyloxycarbonyl-phenylalanine-phenylalanine-diazomethane (Z-Phe-Phe-CHN2).半胱氨酸蛋白酶抑制剂苄氧羰基-苯丙氨酸-丙氨酸-重氮甲烷（Z-Phe-Ala-CHN2）和苄氧羰基-苯丙氨酸-苯丙氨酸-重氮甲烷（Z-Phe-Phe-CHN2）的体外胚胎毒性
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10
Ammonium chloride inhibits basal degradation of newly synthesized collagen in human fetal lung fibroblasts.氯化铵抑制人胎儿肺成纤维细胞中新合成胶原蛋白的基础降解。
Arch Biochem Biophys. 1990 Jan;276(1):125-31. doi: 10.1016/0003-9861(90)90018-t.

引用本文的文献

1
Fluctuations in cell density alter protein markers of multiple cellular compartments, confounding experimental outcomes.细胞密度的波动会改变多个细胞区室的蛋白质标志物，从而混淆实验结果。
PLoS One. 2019 Feb 4;14(2):e0211727. doi: 10.1371/journal.pone.0211727. eCollection 2019.
2
Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes.氨基酸对离体大鼠肝细胞自噬隔离及蛋白质降解的调控
J Cell Biol. 1984 Aug;99(2):435-44. doi: 10.1083/jcb.99.2.435.
3
Distinct proteolytic mechanisms in serum-sufficient and serum-restricted fibroblasts. Transformed 3T3 cells fail to regulate proteolysis in relation to culture density only during serum-sufficiency.血清充足和血清受限的成纤维细胞中不同的蛋白水解机制。仅在血清充足时，转化的3T3细胞在与培养密度相关的情况下无法调节蛋白水解。
Biochem J. 1984 Jul 1;221(1):53-60. doi: 10.1042/bj2210053.
4
Effects of exogenous amines on mammalian cells, with particular reference to membrane flow.外源性胺类对哺乳动物细胞的影响，特别是关于膜流动方面的影响。
Biochem J. 1984 Jan 1;217(1):27-40. doi: 10.1042/bj2170027.
5
Protein turnover in 3T3 cells transformed with the oncogene c-H-ras1.用致癌基因c-H-ras1转化的3T3细胞中的蛋白质周转
Biochem J. 1992 Apr 15;283 ( Pt 2)(Pt 2):427-33. doi: 10.1042/bj2830427.

本文引用的文献

1
The inhibition of macrophage protein turnover by a selective inhibitor of thiol proteinases.巯基蛋白酶选择性抑制剂对巨噬细胞蛋白质周转的抑制作用。
Biochem J. 1980 Feb 15;186(2):385-90. doi: 10.1042/bj1860385.
2
Inhibitors and pathways of hepatocytic protein degradation.肝细胞蛋白降解的抑制剂与途径
Acta Biol Med Ger. 1981;40(10-11):1587-98.
3
Cytoplasmic vacuolation of mouse peritoneal macrophages and the uptake into lysosomes of weakly basic substances.小鼠腹腔巨噬细胞的细胞质空泡化以及弱碱性物质被溶酶体摄取。
J Cell Biol. 1981 Sep;90(3):656-64. doi: 10.1083/jcb.90.3.656.
4
Derangement of regulation of protein degradation in transforming fibroblasts.转化成纤维细胞中蛋白质降解调控的紊乱。
Biosci Rep. 1982 Feb;2(2):107-14. doi: 10.1007/BF01116176.
5
Degradation of intracellular proteins in macrophages and fibroblasts.巨噬细胞和成纤维细胞内蛋白质的降解
Acta Biol Med Ger. 1981;40(10-11):1571-5.
6
Effect of weak bases on the intralysosomal pH in mouse peritoneal macrophages.弱碱对小鼠腹腔巨噬细胞溶酶体内pH值的影响。
J Cell Biol. 1981 Sep;90(3):665-9. doi: 10.1083/jcb.90.3.665.
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Autophagy of metabolically inert substances injected into fibroblasts in culture.
Exp Cell Res. 1981 Sep;135(1):157-66. doi: 10.1016/0014-4827(81)90308-6.
8
Role of individual cathepsins in lysosomal protein digestion as tested by specific inhibitors.通过特异性抑制剂测试的个别组织蛋白酶在溶酶体蛋白消化中的作用。
Biochim Biophys Acta. 1974 Nov 25;370(1):297-307. doi: 10.1016/0005-2744(74)90054-0.
9
Involvement of thiol enzymes in the lysosomal breakdown of native and denatured proteins.硫醇酶参与天然和变性蛋白质的溶酶体分解。
Biochim Biophys Acta. 1973 Jan 24;297(1):98-109. doi: 10.1016/0304-4165(73)90053-6.
10
Direct evidence of importance of lysosomes in degradation of intracellular proteins.溶酶体在细胞内蛋白质降解中重要性的直接证据。
Nature. 1975 Oct 2;257(5525):414-6. doi: 10.1038/257414a0.

正常成纤维细胞接近汇合时蛋白水解的调控。溶酶体系统参与的证据。

The regulation of proteolysis in normal fibroblasts as they approach confluence. Evidence for the participation of the lysosomal system.

作者信息

Cockle S M, Dean R T

出版信息

Biochem J. 1982 Dec 15;208(3):795-800. doi: 10.1042/bj2080795.

DOI:10.1042/bj2080795
PMID:7165734
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1154033/
Abstract

The effect of the lysosomotropic agent NH4Cl and the proteinase inhibitors leupeptin, Z-Phe-Ala-CHN2 (benzyloxycarbonylphenylalanylalanyldiazomethane) and pepstatin on the degradation of intracellular proteins in Swiss 3T3 mouse and normal human fibroblasts in both the exponential and stationary (confluent) growth phases in nutritionally complete conditions was investigated. Inhibitory effects of all four agents on degradation in both growth states were detected. The increase in proteolysis normally occurring as cells approach confluence could be completely blocked by NH4Cl, by Z-Phe-Ala-CHN2, or by pepstatin in the presence of leupeptin. These results suggest that the lysosomal system is responsible for the regulation of proteolysis at confluence and further confirm its role in 'basal' proteolysis in growing cells.

摘要

研究了溶酶体促渗剂氯化铵以及蛋白酶抑制剂亮抑酶肽、Z-苯丙氨酰丙氨酸重氮甲烷和胃蛋白酶抑制剂对营养完全条件下处于指数生长期和平静期（汇合期）的瑞士3T3小鼠及正常人成纤维细胞内蛋白质降解的影响。检测到这四种试剂对两种生长状态下的蛋白质降解均有抑制作用。当细胞接近汇合时正常发生的蛋白水解增加可被氯化铵、Z-苯丙氨酰丙氨酸重氮甲烷或在亮抑酶肽存在时被胃蛋白酶抑制剂完全阻断。这些结果表明溶酶体系统负责汇合时蛋白水解的调节，并进一步证实其在生长细胞“基础”蛋白水解中的作用。