Department of Physical Chemistry, Faculty of Pharmacy, Campus Cartuja, Granada 18071, Spain.
Biochem Soc Trans. 2010 Aug;38(4):914-8. doi: 10.1042/BST0380914.
Two-colour coincidence detection (TCCD) is a form of single-molecule fluorescence developed to sensitively detect and characterize associated biomolecules without any separation, in solution, on the cell membrane and in live cells. In the present short review, we first explain the principles of the method and then describe the application of TCCD to a range of biomedical problems and how this method may be developed further in the future to try to monitor biological processes in live cells.
双色符合检测（TCCD）是一种单分子荧光技术，旨在灵敏地检测和表征溶液、细胞膜和活细胞中相关生物分子，而无需任何分离。在本次简短的综述中，我们首先解释了该方法的原理，然后描述了 TCCD 在一系列生物医学问题中的应用，以及未来如何进一步开发该方法以尝试监测活细胞中的生物过程。
