在单分子水平上通过解旋酶介导的 RNA 结构变化。
Helicase-mediated changes in RNA structure at the single-molecule level.
机构信息
Institute of Inorganic Chemistry, University of Zurich, Zurich, Switzerland.
出版信息
RNA Biol. 2013 Jan;10(1):133-48. doi: 10.4161/rna.23507. Epub 2013 Jan 1.
Abstract
RNA helicases are a diverse group of RNA-dependent ATPases known to play a large number of biological roles inside the cell, such as RNA unwinding, remodeling, export and degradation. Understanding how helicases mediate changes in RNA structure is therefore of fundamental interest. The advent of single-molecule spectroscopic techniques has unveiled with unprecedented detail the interplay of RNA helicases with their substrates. In this review, we describe the characterization of helicase-RNA interactions by single-molecule approaches. State-of-the-art techniques are presented, followed by a discussion of recent advancements in this exciting field.
摘要
RNA 解旋酶是一组多样化的 RNA 依赖的 ATP 酶，已知在细胞内发挥多种生物学作用，如 RNA 解链、重塑、输出和降解。因此，了解解旋酶如何介导 RNA 结构的变化具有根本意义。单分子光谱技术的出现以前所未有的细节揭示了 RNA 解旋酶与其底物的相互作用。在这篇综述中，我们描述了通过单分子方法对解旋酶-RNA 相互作用的表征。介绍了最先进的技术，并讨论了这一令人兴奋的领域的最新进展。
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