十九复合物（NTC）在调控前体 mRNA 剪接过程中剪接体构象和保真度中的功能。
The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicing.
机构信息
Faculty of Life Sciences, Michael Smith Building, The University of Manchester, Manchester M13 9PT, UK.
出版信息
Biochem Soc Trans. 2010 Aug;38(4):1110-5. doi: 10.1042/BST0381110.
Abstract
The NineTeen Complex (NTC) of proteins associates with the spliceosome during pre-mRNA splicing and is essential for both steps of intron removal. The NTC and other NTC-associated proteins are recruited to the spliceosome where they participate in regulating the formation and progression of essential spliceosome conformations required for the two steps of splicing. It is now clear that the NTC is an integral component of active spliceosomes from yeast to humans and provides essential support for the spliceosomal snRNPs (small nuclear ribonucleoproteins). In the present article, we discuss the identification and characterization of the yeast NTC and review recent work in yeast that supports the essential role for this complex in the regulation and fidelity of splicing.
摘要
十九复合物（NTC）的蛋白质与剪接体在 pre-mRNA 剪接过程中结合，对于内含子的去除的两个步骤都是必不可少的。NTC 和其他与 NTC 相关的蛋白质被招募到剪接体，在那里它们参与调节两步剪接所需的关键剪接体构象的形成和进展。现在已经清楚的是，NTC 是从酵母到人活跃剪接体的一个组成部分，并为剪接体 snRNPs（小核核糖核蛋白）提供了必要的支持。在本文中，我们讨论了酵母 NTC 的鉴定和特性，并回顾了酵母中最近的工作，该工作支持了该复合物在剪接的调节和保真度中的重要作用。
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