de Almeida Rogerio Alves, O'Keefe Raymond T
a Faculty of Life Sciences ; The University of Manchester ; Manchester , UK.
RNA Biol. 2015;12(2):109-14. doi: 10.1080/15476286.2015.1008926.
Pre-mRNA splicing is an essential step in gene expression that removes intron sequences efficiently and accurately to produce a mature mRNA for translation. It is the large and dynamic RNA-protein complex called the spliceosome that catalyzes intron removal. To carry out splicing the spliceosome not only needs to assemble correctly with the pre-mRNA but the spliceosome requires extensive remodelling of its RNA and protein components to execute the 2 steps of intron removal. Spliceosome remodelling is achieved through the action of ATPases that target both RNA and proteins to produce spliceosome conformations competent for each step of spliceosome activation, catalysis and disassembly. An increasing amount of research has pointed to the spliceosome associated NineTeen Complex (NTC) of proteins as targets for the action of a number of the spliceosomal ATPases during spliceosome remodelling. In this point-of-view article we present the latest findings on the changes in the NTC that occur following ATPase action that are required for spliceosome activation, catalysis and disassembly. We proposed that the NTC is one of the main targets of ATPase action during spliceosome remodelling required for pre-mRNA splicing.
前体mRNA剪接是基因表达中的一个关键步骤，它能够高效且准确地去除内含子序列，从而产生用于翻译的成熟mRNA。催化内含子去除的是一种名为剪接体的大型动态RNA-蛋白质复合物。为了进行剪接，剪接体不仅需要与前体mRNA正确组装，而且其RNA和蛋白质组分还需要进行广泛重塑，以执行内含子去除的两个步骤。剪接体重塑是通过ATP酶的作用实现的，这些ATP酶作用于RNA和蛋白质，产生适合剪接体激活、催化和解体每个步骤的剪接体构象。越来越多的研究指出，在剪接体重塑过程中，与剪接体相关的蛋白质十九复合物（NTC）是多种剪接体ATP酶作用的靶点。在这篇观点文章中，我们介绍了有关NTC变化的最新研究结果，这些变化发生在剪接体激活、催化和解体所需的ATP酶作用之后。我们提出，NTC是前体mRNA剪接所需的剪接体重塑过程中ATP酶作用的主要靶点之一。
