[从供血者血液的白细胞-血小板层中分离血小板浓缩物及其保存]

[Isolation of thrombocyte concentrates from the leuko-thrombocytic buffy coats of donor blood and their preservation].

作者信息

Agranenko V A, Balezina L V, Kompaniets A M, Volkova R I, Troshina V M, Demicheva M I

出版信息

Gematol Transfuziol. 1991 Mar;36(3):29-32.

PMID:2065954

Abstract

A new method has been developed for platelet concentrate (PC) preparation from the whole blood buffy coats. This method makes possible obtaining leukocyte-poor PC as compared to that prepared from platelet rich plasma. Functional characteristics of PC stored in Soviet plastic bags at room temperature (22 degrees C) under continuous agitation at circular rotator were studied during 7 days. Satisfactory maintenance of functional activity was noted during 5 days of PC storage. The method of PC preparation from the whole blood buffy coats can be also used for manual plateletpheresis of a single donor.

摘要

已开发出一种从全血白膜层制备浓缩血小板（PC）的新方法。与从富血小板血浆制备的PC相比，该方法能够获得白细胞含量低的PC。研究了在室温（22摄氏度）下，于循环旋转器上持续搅拌的条件下，储存在苏联塑料袋中的PC在7天内的功能特性。在PC储存的5天内，观察到其功能活性得到了令人满意的维持。从全血白膜层制备PC的方法也可用于单个供体的手工血小板采集。