Pietersz R N, Loos J A, Reesink H W
Blut. 1987 Apr;54(4):201-6. doi: 10.1007/BF00594194.
High speed centrifugation allows separation of whole blood into cell free plasma, a buffy coat and leukocyte poor red cells. The buffy coat can be used for the preparation of platelet concentrates. High lactate production at 22 degrees C requires storage of the buffy coat at 4 degrees C. Survival in vivo of platelet concentrates prepared from buffy coats stored at 4 degrees C for 48 h (BC-PC) was compared with the survival in vivo of platelet concentrates from platelet rich plasma stored at 22 degrees C for 48 h (PRP-PC). Both methods were studied in the same healthy volunteers (n = 8) using 51Cr labeled autologous platelets. The mean +/- SD recovery 15 min after reinfusion of the BC-PC was 30.5% +/- 13.3% and for PRP-PC 41.4% +/- 7.9% (p less than 0.0001). The survival in vivo for BC-PC was 2.4 days +/- 0.4 days and for PRP-PC 7.0 days +/- 1.4 days (p less than 0.0001). Since the survival in vivo is significantly less for platelets derived from the buffy coat stored at 4 degrees C, we advocate storage of platelets at 22 degrees C.
高速离心可将全血分离为无细胞血浆、白膜层和贫白细胞红细胞。白膜层可用于制备浓缩血小板。22℃时乳酸产生量高，因此白膜层需在4℃储存。将在4℃储存48小时的白膜层制备的浓缩血小板（BC - PC）在体内的存活情况与在22℃储存48小时的富血小板血浆制备的浓缩血小板（PRP - PC）在体内的存活情况进行了比较。在相同的健康志愿者（n = 8）中使用51Cr标记的自体血小板对两种方法进行了研究。回输BC - PC后15分钟的平均±标准差回收率为30.5%±13.3%，PRP - PC为41.4%±7.9%（p＜0.0001）。BC - PC在体内的存活时间为2.4天±0.4天，PRP - PC为7.0天±1.4天（p＜0.0001）。由于在4℃储存的白膜层来源的血小板在体内的存活时间明显较短，我们主张将血小板储存在22℃。
