Rácz Z, Baróti C
National Institute of Hematology, Blood Transfusion and Immunology, Budapest, Hungary.
Vox Sang. 1995;68(3):160-3. doi: 10.1111/j.1423-0410.1995.tb03918.x.
Platelet concentrates (PCs) were prepared from single buffy coats derived from fresh blood and from blood units stored overnight, as well as from buffy coats that were stored overnight. The platelet yield from overnight-stored buffy coats was similar to that of fresh blood or overnight-stored blood. PCs were stored at 20-24 degrees C and on day 5 of storage, platelet aggregation with ADP was tested both at 37 and 25 degrees C. Stored platelets aggregated better at 25 degrees C than 37 degrees C. The maximal, aggregation (10 microM ADP) of stored platelets from overnight-stored buffy coats was 46 +/- 23% (n = 30), while that of stored platelets prepared either from fresh or overnight-stored blood was 27 +/- 21% (n = 29) and 22 +/- 15% (n = 29), respectively. Extracellular lactate dehydrogenase and ammonia levels, as well as elastase activity were similar in stored PCs of different origin. Our conclusion is that PCs prepared from overnight-stored buffy coat might also be suitable for storage and clinical use. In vivo studies are needed to confirm our findings.
血小板浓缩物（PCs）由新鲜血液的单个白膜层、过夜储存的血液单位以及过夜储存的白膜层制备而成。过夜储存白膜层的血小板产量与新鲜血液或过夜储存血液的产量相似。PCs在20 - 24摄氏度下储存，在储存第5天，在37摄氏度和25摄氏度下测试血小板与ADP的聚集情况。储存的血小板在25摄氏度下比在37摄氏度下聚集得更好。过夜储存白膜层的储存血小板的最大聚集率（10微摩尔ADP）为46±23%（n = 30），而由新鲜或过夜储存血液制备的储存血小板的最大聚集率分别为27±21%（n = 29）和22±15%（n = 29）。不同来源的储存PCs中的细胞外乳酸脱氢酶和氨水平以及弹性蛋白酶活性相似。我们的结论是，由过夜储存白膜层制备的PCs可能也适用于储存和临床使用。需要进行体内研究来证实我们的发现。
