Department of Biomedical Engineering, Linköping Biomedical Optics Group, Linköping University, 58185 Linköping, Sweden.
J Biophotonics. 2011 Apr;4(4):268-76. doi: 10.1002/jbio.201000048.
The aim of this study was to compare a previously used light transport model (I) comprising the chromophores hemo- and myoglobin, fat, and water, with two extended models, where the chromophores of cytochrome aa3, methemo- and metmyoglobin are added (model II), and in addition, accounting for an inhomogenous hemoglobin distribution (model III). The models were evaluated using calibrated diffuse reflectance spectroscopy measurements on the human myocardium. Model II proved a significantly better spectral fitting, especially in the wavelength ranges corresponding to prominent absorption characteristics for the added chromophores. Model III was significantly better than model II and displayed a markedly higher tissue fraction and saturation of hemo- and myoglobin. The estimated tissue chromophore fractions, saturation and oxidation levels, were in agreement with other studies, demonstrating the potential of diffuse reflectance spectroscopy measurements for evaluating open heart surgery. However, the choice of chromophores and vessel packaging effects in the light transport model has a major effect on the results.
本研究旨在比较先前使用的光传输模型（I），该模型包含红血球和肌红蛋白、脂肪和水等发色团，以及两个扩展模型，其中添加细胞色素 aa3、高铁肌红蛋白和正铁肌红蛋白的发色团（模型 II），并考虑到血红蛋白的非均匀分布（模型 III）。使用校准的漫反射光谱测量法对人体心肌进行了模型评估。模型 II 证明了更好的光谱拟合，特别是在与添加的发色团的突出吸收特征相对应的波长范围内。模型 III 明显优于模型 II，并且显示出更高的组织分数和血红蛋白饱和度。估计的组织发色团分数、饱和度和氧化水平与其他研究一致，表明漫反射光谱测量在评估心脏直视手术方面具有潜力。然而，光传输模型中发色团的选择和血管包装效果对结果有重大影响。
