Brusevold Ingvild J, Søland Tine M, Khuu Cuong, Christoffersen Thoralf, Bryne Magne
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
Eur J Oral Sci. 2010 Aug;118(4):342-9. doi: 10.1111/j.1600-0722.2010.00747.x.
Met, the hepatocyte growth factor receptor, is important in transducing signals for tumour growth and metastasis. The aim of this study was to examine the pattern of Met expression and its value as a prognostic factor in oral squamous cell carcinomas (OSCCs). The material consisted of 53 OSCCs and five healthy controls from normal oral mucosa supplied with cell lines, 10 organotypic models supplied with oral cancer cells, and three organotypic models supplied with normal keratinocytes. Met protein expression was assessed by immunohistochemistry and western blotting. Met expression was scarce and limited to the basal layer in normal oral mucosa, but was more extensive in the tumours. Cytoplasmic expression of Met was found in the majority of the tumours, and nuclear expression was found in 72%, including a high fraction of the cells located at the invasive front. Organotypic models with normal or malignant oral cells yielded principally similar results as in the mucosa and the cancers, respectively. A smaller amount of Met immunoreactivity was detected, by western blotting, in the nuclear fraction of cultured oral cancer cells. In conclusion, Met was upregulated in OSCCs and was also found in the nucleus. However, Met was not a marker for prognosis in this study.
肝细胞生长因子受体Met在转导肿瘤生长和转移信号方面具有重要作用。本研究旨在探讨Met在口腔鳞状细胞癌（OSCC）中的表达模式及其作为预后因素的价值。研究材料包括53例OSCC、5例来自正常口腔黏膜并伴有细胞系的健康对照、10例含有口腔癌细胞的器官型模型以及3例含有正常角质形成细胞的器官型模型。通过免疫组织化学和蛋白质印迹法评估Met蛋白表达。Met在正常口腔黏膜中的表达稀少且局限于基底层，但在肿瘤中表达更为广泛。大多数肿瘤中发现Met的细胞质表达，72%的肿瘤中发现Met的核表达，包括位于侵袭前沿的大部分细胞。含有正常或恶性口腔细胞的器官型模型分别在黏膜和癌症中产生了大致相似的结果。通过蛋白质印迹法在培养的口腔癌细胞的核组分中检测到较少量的Met免疫反应性。总之，Met在OSCC中上调且也存在于细胞核中。然而，在本研究中Met并非预后标志物。
