Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti - Sezione di Fisiologia Veterinaria, Università degli Studi di Parma, Parma, Italy.
J Anim Physiol Anim Nutr (Berl). 2010 Dec;94(6):e374-82. doi: 10.1111/j.1439-0396.2010.01025.x.
Soya and soybean products used in swine feeding contain genistein, a non-steroidal phyto-oestrogen which has been demonstrated to influence endocrine functions. This observation leads us to design this study to evaluate the effect of genistein on swine granulosa cell steroidogenesis and proliferation. In the attempt to unravel the genistein signal transduction mechanisms, we verified the effect of lavendustin, a Tyrosine Kinase (TK) inhibitor, and the potential involvement of NO/cGMP pathway. Finally, as angiogenesis is essential for follicle development, we tested the effect of the phyto-oestrogen on vascular endothelial growth factor production and on granulosa cell redox status, because free-radical species modulate neovascularization. Our data provide evidence that genistein interferes with granulosa cell steroidogenesis while it does not modulate cell growth: this effect could be at least partially produced by inhibiting TK-dependent signalling systems. On the contrary, NO/cGMP pathway or vascular endothelial growth factor production can be excluded as signalling mechanism involved in phyto-oestrogen effects. Remarkably, genistein stimulates hydrogen peroxide production thus potentially inhibiting follicular angiogenesis. Collectively, these results suggest that genistein consumption could potentially negatively impact swine reproductive function.
大豆和大豆制品在猪的饲养中被使用，其中含有染料木黄酮，一种非甾体植物雌激素，已被证明会影响内分泌功能。这一观察结果促使我们设计了这项研究，以评估染料木黄酮对猪颗粒细胞类固醇生成和增殖的影响。为了阐明染料木黄酮的信号转导机制，我们验证了酪蛋白激酶（TK）抑制剂 lavendustin 的作用，以及 NO/cGMP 途径的潜在参与。最后，由于血管生成对于卵泡发育至关重要，我们测试了植物雌激素对血管内皮生长因子产生和颗粒细胞氧化还原状态的影响，因为自由基种类调节新血管生成。我们的数据提供了证据，表明染料木黄酮干扰颗粒细胞类固醇生成，而不调节细胞生长：这种作用至少可以部分通过抑制 TK 依赖性信号系统产生。相反，NO/cGMP 途径或血管内皮生长因子的产生可以排除作为涉及植物雌激素作用的信号机制。值得注意的是，染料木黄酮刺激过氧化氢的产生，从而可能抑制卵泡的血管生成。总的来说，这些结果表明，染料木黄酮的摄入可能会对猪的生殖功能产生负面影响。
