Basini G, Bussolati S, Baioni L, Grasselli F
Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti, Sezione di Fisiologia Veterinaria, Via del Taglio 10, Università degli Studi di Parma, Parma, Italy.
Domest Anim Endocrinol. 2009 Jul;37(1):30-6. doi: 10.1016/j.domaniend.2009.01.005. Epub 2009 Mar 22.
Gossypol is a polyphenol isolated from the seed, roots and stem of cotton plant (Gossypium sp.) It has been associated with adverse effects on female reproduction, but recently also shown having promising effects against several malignancies. Its mechanisms of action are however still not fully understood. This study was therefore conducted to investigate the effect of 5 or 25 microg/mL gossypol on swine granulosa cell steroidogenic activity, redox status and Vascular Endothelial Growth Factor (VEGF) production. Study demonstrated that gossypol significantly (P<0.001) inhibited granulosa cell estradiol 17beta and progesterone production, an effect that could be at least partially mediated by an increase (P<0.05) of nitric oxide and superoxide anion production as a consequence of superoxide dismutase inhibition. Moreover, gossypol stimulates (P<0.001) VEGF production. In conclusion, study has demonstrated effects of gossypol on swine granulosa cell function in vitro. Effects on female swine fertility can not be excluded.
棉酚是从棉花植株（棉属）的种子、根和茎中分离出的一种多酚。它与对雌性生殖的不良影响有关，但最近也显示出对几种恶性肿瘤有潜在的治疗作用。然而，其作用机制仍未完全明确。因此，本研究旨在探讨5或25微克/毫升棉酚对猪颗粒细胞类固醇生成活性、氧化还原状态和血管内皮生长因子（VEGF）产生的影响。研究表明，棉酚显著（P<0.001）抑制颗粒细胞17β-雌二醇和孕酮的产生，这一作用至少部分是由于超氧化物歧化酶抑制导致一氧化氮和超氧阴离子产生增加（P<0.05）所介导的。此外，棉酚刺激（P<0.001）VEGF的产生。总之，本研究证明了棉酚对体外猪颗粒细胞功能的影响。不能排除其对雌性猪生育能力的影响。
