Department of Comparative Biosciences, University of Illinois, 2001 S. Lincoln Ave, Urbana, IL, 61802, United States.
Department of Food Science and Human Nutrition, University of Illinois, 905 S. Goodwin, Urbana, IL, 61801, United States.
Reprod Toxicol. 2017 Dec;74:174-180. doi: 10.1016/j.reprotox.2017.09.014. Epub 2017 Sep 29.
Genistein is a phytoestrogen found in soy and soy-based products. Previously, we found that genistein adversely affected estradiol levels and follicle growth in vitro. Proper hormone production and follicle growth are key regulators of normal fertility. Therefore, we hypothesized that genistein adversely affects female fertility and pregnancy outcomes. To test this hypothesis, we dosed sexually mature female CD-1 mice (35days) with 0, 300, 500, or 1000ppm genistein for 30, 60, 150, and 240days. At the end of the dosing periods, we measured mating rate, pregnancy rate, fertility rate, gestation time, parturition time, pup mortality, litter size, average pup weight, and estradiol and progesterone levels. We found that chronic, preconception exposure to genistein affects gestation time, parturition time, litter size, pup weight, and pup mortality. Additionally, genistein exposure for 240days appears to have a protective effect on fertility rate, but does not affect hormone levels in vivo.
染料木黄酮是一种存在于大豆及其制品中的植物雌激素。此前，我们发现染料木黄酮会对体外雌二醇水平和卵泡生长产生不利影响。适当的激素产生和卵泡生长是正常生育能力的关键调节因素。因此，我们假设染料木黄酮会对女性生育能力和妊娠结局产生不利影响。为了验证这一假设，我们用 0、300、500 或 1000ppm 的染料木黄酮对性成熟的 CD-1 小鼠（35 天）进行了为期 30、60、150 和 240 天的处理。在给药期结束时，我们测量了交配率、妊娠率、生育率、妊娠期、分娩时间、幼仔死亡率、窝仔数、平均幼仔体重以及雌二醇和孕酮水平。我们发现，慢性、孕前暴露于染料木黄酮会影响妊娠期、分娩时间、窝仔数、幼仔体重和幼仔死亡率。此外，暴露于染料木黄酮 240 天似乎对生育率有保护作用，但不会影响体内激素水平。
