使用大肠杆菌β-葡萄糖醛酸酶基因作为报告基因来研究拟杆菌属启动子。
Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters.
作者信息
Feldhaus M J, Hwa V, Cheng Q, Salyers A A
机构信息
Department of Microbiology, University of Illinois, Urbana 61801.
出版信息
J Bacteriol. 1991 Jul;173(14):4540-3. doi: 10.1128/jb.173.14.4540-4543.1991.
Abstract
We have constructed transcriptional fusion vectors for use in Bacteroides spp., a genus of gram-negative obligate anaerobes found in high numbers in the human colon. The reporter group in these vectors is a promoterless beta-glucuronidase gene from Escherichia coli (uidA). Two of the vectors (pMJF-2 and pMJF-3) replicate in Bacteroides spp. The third, pCQW-1, does not replicate in Bacteroides spp. and can be used to introduce E. coli beta-glucuroindase fusions into the Bacteroides chromosome.
摘要
我们构建了用于拟杆菌属的转录融合载体，拟杆菌属是革兰氏阴性专性厌氧菌，在人类结肠中大量存在。这些载体中的报告基团是来自大肠杆菌的无启动子β-葡萄糖醛酸酶基因（uidA）。其中两个载体（pMJF-2和pMJF-3）可在拟杆菌属中复制。第三个载体pCQW-1不能在拟杆菌属中复制，可用于将大肠杆菌β-葡萄糖醛酸酶融合基因导入拟杆菌属染色体。
相似文献
1
Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters.
J Bacteriol. 1991 Jul;173(14):4540-3. doi: 10.1128/jb.173.14.4540-4543.1991.
2
Plasmids with the uidA reporter gene for the detection of promoters and transcription signals.
Mol Gen Genet. 1988 May;212(2):390-2. doi: 10.1007/BF00334714.
3
Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers.
Gene. 1994 Jan 28;138(1-2):1-7. doi: 10.1016/0378-1119(94)90776-5.
4
Development of a bifunctional xylosidase/arabinosidase gene as a reporter gene for the gram-negative anaerobes Bacteroides and Porphyromonas, and Escherichia coli.
Curr Microbiol. 1997 Nov;35(5):282-6. doi: 10.1007/s002849900255.
5
Heterologous gene expression in Bacteroides fragilis.
Plasmid. 1992 Mar;27(2):141-54. doi: 10.1016/0147-619x(92)90014-2.
6
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447-51. doi: 10.1073/pnas.83.22.8447.
7
Use of the Escherichia coli beta-glucuronidase (gusA) gene as a reporter gene for analyzing promoters in lactic acid bacteria.
Appl Environ Microbiol. 1994 Feb;60(2):587-93. doi: 10.1128/aem.60.2.587-593.1994.
8
Genetic analysis of the minimal replicon of plasmid pIP417 and comparison with the other encoding 5-nitroimidazole resistance plasmids from Bacteroides spp.
Plasmid. 1995 Sep;34(2):132-43. doi: 10.1006/plas.1995.9994.
9
Recent advances in Bacteroides genetics.
Crit Rev Microbiol. 1987;14(1):49-71. doi: 10.3109/10408418709104435.
10
Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.
Appl Environ Microbiol. 2006 Nov;72(11):7401-5. doi: 10.1128/AEM.01611-06. Epub 2006 Sep 22.
引用本文的文献
1
Mutagenesis of the gene involved in alteration of outer membrane composition.
Iran J Microbiol. 2019 Feb;11(1):67-74.
2
Defining the bacteroides ribosomal binding site.
Appl Environ Microbiol. 2013 Mar;79(6):1980-9. doi: 10.1128/AEM.03086-12. Epub 2013 Jan 18.
3
The excision proteins of CTnDOT positively regulate the transfer operon.
Plasmid. 2013 Mar;69(2):172-9. doi: 10.1016/j.plasmid.2012.12.001. Epub 2012 Dec 10.
4
Characterization of the Bacteroides CTnDOT regulatory protein RteC.
J Bacteriol. 2011 Jan;193(1):91-7. doi: 10.1128/JB.01015-10. Epub 2010 Oct 29.
5
Tetracycline-associated transcriptional regulation of transfer genes of the Bacteroides conjugative transposon CTnDOT.
J Bacteriol. 2009 Oct;191(20):6374-82. doi: 10.1128/JB.00739-09. Epub 2009 Aug 21.
6
Regulation of excision genes of the Bacteroides conjugative transposon CTnDOT.
J Bacteriol. 2005 Aug;187(16):5732-41. doi: 10.1128/JB.187.16.5732-5741.2005.
7
Translational control of tetracycline resistance and conjugation in the Bacteroides conjugative transposon CTnDOT.
J Bacteriol. 2005 Apr;187(8):2673-80. doi: 10.1128/JB.187.8.2673-2680.2005.
8
Regulation of a Bacteroides operon that controls excision and transfer of the conjugative transposon CTnDOT.
J Bacteriol. 2004 May;186(9):2548-57. doi: 10.1128/JB.186.9.2548-2557.2004.
9
Intracellular induction of Listeria monocytogenes actA expression.
Infect Immun. 2002 Mar;70(3):1087-96. doi: 10.1128/IAI.70.3.1087-1096.2002.
10
New regulatory gene that contributes to control of Bacteroides thetaiotaomicron starch utilization genes.
J Bacteriol. 2001 Dec;183(24):7198-205. doi: 10.1128/JB.183.24.7198-7205.2001.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
Location and nucleotide sequence of the transfer origin of the broad host range plasmid RK2.
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3595-8. doi: 10.1073/pnas.80.12.3595.
3
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447-51. doi: 10.1073/pnas.83.22.8447.
4
Mobilization of Bacteroides plasmids by Bacteroides conjugal elements.
J Bacteriol. 1988 Mar;170(3):1319-24. doi: 10.1128/jb.170.3.1319-1324.1988.
5
Complete nucleotide sequence and transcription of ermF, a macrolide-lincosamide-streptogramin B resistance determinant from Bacteroides fragilis.
J Bacteriol. 1986 Nov;168(2):523-33. doi: 10.1128/jb.168.2.523-533.1986.
6
Use of targeted insertional mutagenesis to determine whether chondroitin lyase II is essential for chondroitin sulfate utilization by Bacteroides thetaiotaomicron.
J Bacteriol. 1986 Jun;166(3):966-71. doi: 10.1128/jb.166.3.966-971.1986.
7
Development and use of cloning systems for Bacteroides fragilis: cloning of a plasmid-encoded clindamycin resistance determinant.
J Bacteriol. 1985 Oct;164(1):294-301. doi: 10.1128/jb.164.1.294-301.1985.
8
Nucleotide sequence analysis of Tn4551: use of ermFS operon fusions to detect promoter activity in Bacteroides fragilis.
J Bacteriol. 1987 Oct;169(10):4589-96. doi: 10.1128/jb.169.10.4589-4596.1987.
9
A cryptic 65-kilobase-pair transposonlike element isolated from Bacteroides uniformis has homology with Bacteroides conjugal tetracycline resistance elements.
J Bacteriol. 1990 Apr;172(4):1694-702. doi: 10.1128/jb.172.4.1694-1702.1990.
Zotero 插件