高通量蛋白质结晶。
High-throughput protein crystallization.
机构信息
Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK.
出版信息
Adv Protein Chem Struct Biol. 2009;77:1-22. doi: 10.1016/S1876-1623(09)77001-4. Epub 2009 Nov 28.
Abstract
Structural genomics projects have led to great progress in the field of structural biology. Considerable advances have been made in the automation of all stages of the pipeline from clone to structure. This chapter focuses on crystallization that is one of the major bottlenecks in this pipeline. It discusses new developments and describes a variety of techniques for high-throughput screening and optimizing of conditions for crystallization.
摘要
结构基因组学项目在结构生物学领域取得了重大进展。从克隆到结构的整个流水线的自动化都取得了相当大的进展。本章重点介绍结晶，这是该流水线的主要瓶颈之一。它讨论了新的发展，并描述了高通量筛选和优化结晶条件的各种技术。
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