因饲料导致的毛皮动物肉毒梭菌中毒危险。

Fur animal botulism hazard due to feed.

机构信息

Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, Finland.

出版信息

Res Vet Sci. 2011 Jun;90(3):412-8. doi: 10.1016/j.rvsc.2010.06.024. Epub 2010 Jul 20.

DOI:10.1016/j.rvsc.2010.06.024

PMID:20663530

Abstract

To assess the botulism hazard in fur animal feed production, 236 fur animal feed components and feed samples were analysed for Clostridium botulinum by detecting BoNT-encoding genes (botA, botB, botC, botE or botF) by PCR and for sulphite-reducing clostridia (SRC) by iron sulphite agar. The quality of the hazard analysis of critical control points (HACCP) -based in-house control system (IHCS) was evaluated with respect to botulism risk in feed plants (n=32). The overall prevalence of C. botulinum was 13% in different feed components and 5% in feed. The estimated MPN count of C. botulinum in feed components was 6.4 × 10(3)/kg at the highest and was shown to poorly correlate with SRC count. The critical control points in IHCSs were variable, and control limits were improperly set in most feed-producing plants. C. botulinum possesses a persistent safety hazard for fur animals by feed production, and control practices should be reassessed.

摘要

为了评估毛皮动物饲料生产中的肉毒梭菌危害，通过 PCR 检测 BoNT 编码基因（botA、botB、botC、botE 或 botF）和亚硫酸铁琼脂检测亚硫酸盐还原梭菌（SRC），对 236 种毛皮动物饲料成分和饲料样品进行了分析。采用危害分析关键控制点（HACCP）的内部控制系统（IHCS），对饲料厂（n=32）的肉毒梭菌风险进行了评价。在不同的饲料成分和饲料中，C. botulinum 的总流行率分别为 13%和 5%。在饲料成分中，C. botulinum 的估计 MPN 计数最高可达 6.4×10(3)/kg，与 SRC 计数相关性较差。IHCS 中的关键控制点各不相同，而且大多数饲料生产厂的控制限值设置不当。C. botulinum 通过饲料生产对毛皮动物存在持续的安全危害，应重新评估控制措施。