Grenda Tomasz, Goldsztejn Magdalena, Kwiatek Krzysztof, Kozak Beata, Grenda Anna
Department of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, 24-100 Puławy, Poland.
Department of Pneumology, Oncology and Allergology, Medical University of Lublin, 20-954, Lublin, Poland.
J Vet Res. 2022 May 31;66(2):189-197. doi: 10.2478/jvetres-2022-0026. eCollection 2022 Jun.
The aim of the study was to present cases of botulism in animals found in Poland in 2019-2021. The analytical laboratory diagnosis and difficulties that occurred in the interpretation of the results are described.
From 2019 to 2021, samples of serum, intestinal content, liver, spleen, kidney, faeces, wet feed, dry feed, ensilage, water and mixed samples of internal organs associated with 10 suspected animal botulism cases were sent to the National Veterinary Research Institute. Samples were analysed using a mouse bioassay and culture methods in combination with and gene detection.
Among the ten putative botulism cases, only four (40%) were confirmed in the laboratory on the basis of the detection of botulinum toxin (BoNT) or the or genes. The remaining six (60%) were determined as probable despite observable characteristic clinical signs.
The diagnosis of botulism in animals is a very difficult task, made so by the heterogeneity of strains and possible loss of toxinogenicity during laboratory processing or the potential degradation of toxins. Laboratory diagnosis is a complex and problematic process which should utilise different prescribed methods for specific types of sample.
本研究的目的是介绍2019 - 2021年在波兰发现的动物肉毒中毒病例。描述了分析实验室诊断以及结果解读中遇到的困难。
2019年至2021年期间，与10例疑似动物肉毒中毒病例相关的血清、肠内容物、肝脏、脾脏、肾脏、粪便、湿饲料、干饲料、青贮饲料、水和混合内脏样本被送往国家兽医研究所。样本采用小鼠生物测定法和培养方法，并结合 和 基因检测进行分析。
在这10例疑似肉毒中毒病例中，只有4例（40%）基于肉毒毒素（BoNT）或 或 基因的检测在实验室得到确诊。其余6例（60%）尽管有明显的特征性临床症状，但仍被判定为可能病例。
动物肉毒中毒的诊断是一项非常困难的任务，由于 菌株的异质性以及实验室处理过程中可能出现的产毒性丧失或毒素的潜在降解而变得如此。实验室诊断是一个复杂且有问题的过程，应针对特定类型的样本采用不同的规定方法。
