Mackinnon E S, El-Sohemy A, Rao A V, Rao L G
Department of Medicine, Division of Endocrinology and Metabolism, St. Michael's Hospital, Toronto, Ont., Canada.
J Nutrigenet Nutrigenomics. 2010;3(1):1-8. doi: 10.1159/000316636. Epub 2010 Jul 28.
BACKGROUND/AIMS: Polymorphisms of the paraoxonase 1 (PON1) enzyme affect the ability to protect LDL from oxidation. Oxidative stress is a risk factor for osteoporosis and antioxidants may be beneficial for prevention. The aim of this study was to determine whether PON1 genotypes modified the association between lycopene and bone turnover markers and oxidative stress parameters.
Blood samples from 107 women 25-70 years of age were analyzed for serum carotenoid concentrations, bone-specific alkaline phosphatase (BAP), N-telopeptide of type I collagen (NTx) and oxidative stress parameters. Subjects were genotyped for the 172T→A and 584A→G polymorphisms of PON1.
The 172T→A polymorphism modified the association between lycopene and NTx (p < 0.05 for interaction). In the 172TT genotype, high serum lycopene was associated with decreased NTx (p < 0.05). The 584A→G polymorphism modified the association between lycopene and BAP (p < 0.05 for interaction). Additionally, in participants with the 584GG genotype, high serum lycopene was associated with high TBA-reactive substances (p < 0.05).
These findings show that PON1 polymorphisms modify the association between serum concentrations of lycopene and oxidative stress parameters and bone turnover markers and may, therefore, moderate the risk of osteoporosis.
背景/目的：对氧磷酶1（PON1）的多态性会影响其保护低密度脂蛋白免受氧化的能力。氧化应激是骨质疏松症的一个风险因素，抗氧化剂可能有助于预防。本研究的目的是确定PON1基因型是否会改变番茄红素与骨转换标志物及氧化应激参数之间的关联。
对107名年龄在25至70岁之间的女性的血液样本进行分析，检测血清类胡萝卜素浓度、骨特异性碱性磷酸酶（BAP）、I型胶原N端肽（NTx）及氧化应激参数。对受试者的PON1基因172T→A和584A→G多态性进行基因分型。
172T→A多态性改变了番茄红素与NTx之间的关联（交互作用p<0.05）。在172TT基因型中，高血清番茄红素与NTx降低相关（p<0.05）。584A→G多态性改变了番茄红素与BAP之间的关联（交互作用p<0.05）。此外，在584GG基因型的参与者中，高血清番茄红素与高TBA反应性物质相关（p<0.05）。
这些发现表明，PON1多态性改变了血清番茄红素浓度与氧化应激参数及骨转换标志物之间的关联，并因此可能会降低骨质疏松症的风险。
