Bar-Shavit R, Laub O, Aloni Y
J Gen Virol. 1978 May;39(2):357-60. doi: 10.1099/0022-1317-39-2-357.
Exhaustion type hybridization was used to measure the amount of nuclear virus RNA complementary to the early (E) and late (L) polyoma virus DNA strands. At 36 h after infection between 2.5 and 7.3% of the newly synthesized virus RNA was complementary to the E-strand (-strand) and between 92.7 and 97.5% was complementary to the L-strand (+strand). This proportion was independent of the labelling time, indicating similar accumulation of the E- and L-RNA transcripts in the nucleus. The nuclear E- and L-RNA transcripts sedimented in a similar manner through sucrose gradients.
采用耗尽型杂交法来测定与多瘤病毒早期（E）和晚期（L）DNA链互补的核病毒RNA的量。感染后36小时，新合成的病毒RNA中2.5%至7.3%与E链（负链）互补，92.7%至97.5%与L链（正链）互补。该比例与标记时间无关，表明E-RNA和L-RNA转录本在细胞核中的积累情况相似。核E-RNA和L-RNA转录本通过蔗糖梯度离心时沉降方式相似。
