从东海放线菌中克隆和体外鉴定 dTDP-6-脱氧-L-塔洛糖生物合成基因，该基因编码的 dTDP-6-脱氧-L--lyxo-4-己酮糖还原酶合成 dTDP-6-脱氧-L-塔洛糖。

Cloning and in vitro characterization of dTDP-6-deoxy-L-talose biosynthetic genes from Kitasatospora kifunensis featuring the dTDP-6-deoxy-L-lyxo-4-hexulose reductase that synthesizes dTDP-6-deoxy-L-talose.

机构信息

Department of Biological Science, Myongji University, Yongin, Republic of Korea.

出版信息

Carbohydr Res. 2010 Sep 3;345(13):1958-62. doi: 10.1016/j.carres.2010.07.004.

DOI:10.1016/j.carres.2010.07.004

PMID:20667525

Abstract

Kitasatospora kifunensis, the talosin producer, was used as a source for the dTDP-6-deoxy-l-talose (dTDP-6dTal) biosynthetic gene cluster, serving as a template for four recombinant proteins of RmlA(Kkf), RmlB(Kkf), RmlC(Kkf), and Tal, which complete the biosynthesis of dTDP-6dTal from dTTP, alpha-d-glucose-1-phosphate, and NAD(P)H. The identity of dTDP-6dTal was validated using (1)H and (13)C NMR spectroscopy. K. kifunensistal and tll, the known dTDP-6dTal synthase gene of Actinobacillus actinomycetemcomitans origin, have low sequence similarity and are distantly related within the NDP-6-deoxy-4-ketohexose reductase family, providing an example of the genetic diversity within the dTDP-6dTal biosynthetic pathway.

摘要

金褐链霉菌（Kitasatospora kifunensis）是塔洛辛的产生菌，被用作 dTDP-6-去氧-l-塔洛糖（dTDP-6dTal）生物合成基因簇的来源，作为 RmlA（Kkf）、RmlB（Kkf）、RmlC（Kkf）和 Tal 这四种重组蛋白的模板，它们完成了 dTDP-6dTal 从 dTTP、α-d-葡萄糖-1-磷酸和 NAD（P）H 的生物合成。使用 (1)H 和 (13)C NMR 光谱验证了 dTDP-6dTal 的身份。金褐链霉菌 tal 和 tll，是放线菌 Actinobacillus actinomycetemcomitans 中已知的 dTDP-6dTal 合酶基因，它们的序列相似度较低，在 NDP-6-去氧-4-酮己糖还原酶家族中亲缘关系较远，为 dTDP-6dTal 生物合成途径中的遗传多样性提供了一个例证。