Department of Human Anatomical Sciences, University of Bologna, via Irnerio, 48-40126 Bologna, Italy.
Int J Toxicol. 2010 Sep-Oct;29(5):523-31. doi: 10.1177/1091581810375003. Epub 2010 Jul 28.
The purpose of this study was to evaluate the cytotoxicity of low doses and long-term exposure to 2-hydroxyethylmethacrylate (HEMA) on the protein expression of human gingival fibroblasts (HGFs). Human gingival fibroblasts were exposed to different concentrations of HEMA ranging from 0.5 mmol/L to 3 mmol/L for periods of time from 72 hours to 2 weeks. A significant decrease in the expression of procollagen α1 type I protein was observed 72 hours after treatment of cells with 3 mmol/L HEMA. Although low concentrations of the monomer after 2 weeks of exposure to HEMA did not appear to induce any marked changes in the morphology or viability of cells, the expression of procollagen α1 type I protein and its messenger RNA (mRNA) markedly decreased. In conclusion, our data demonstrated that cell viability and morphology assays could be deficient parameters in evaluating the biocompatibility of dental resin materials.
本研究旨在评估低剂量和长期暴露于 2-羟乙基甲基丙烯酸酯（HEMA）对人牙龈成纤维细胞（HGFs）蛋白表达的细胞毒性。人牙龈成纤维细胞分别暴露于浓度范围为 0.5mmol/L 至 3mmol/L 的 HEMA 中，暴露时间从 72 小时至 2 周不等。在以 3mmol/L HEMA 处理细胞 72 小时后，观察到 I 型前胶原α1 蛋白的表达显著下降。尽管在暴露于 HEMA 2 周后低浓度的单体似乎没有引起细胞形态或活力的任何明显变化，但 I 型前胶原α1 蛋白及其信使 RNA（mRNA）的表达明显下降。总之，我们的数据表明，细胞活力和形态测定可能是评估牙科树脂材料生物相容性的缺陷参数。
