Banava Sepideh, Najibfard Kaveh, Garcia-Godoy Franklin, Saghiri Mohammad Ali, Ghahremani Mohammad Hossein, Ostad Naser
Assistant Professor, Department of Dental Materials, Azad University, Dental Branch, Tehran, Iran.
Private Practice, San Antonio, Texas.
J Dent Res Dent Clin Dent Prospects. 2015 Summer;9(3):151-8. doi: 10.15171/joddd.2015.029. Epub 2015 Sep 16.
Background and aims. The aim of this study was to evaluate the effect of dilution and curing methods of an etch-and-rinse adhesive and a self-etching primer from the same manufacturer at early exposure time on cytotoxicity of primary human gingival fibroblasts. Materials and methods. Primary human gingival fibroblasts were exposed to different dilutions of Adper Single Bond (ASB) and Adper Prompt L-Pop (APL) (3M ESPE, USA). They were evaluated in unpolymerized mode for 20 s, 5 min and 24 h and in polymerized mode for 24 h and 48 h. Cytotoxicity was evaluated using three cytotoxic tests (MTT, cell counting and DNA condensation). Data was analyzed by a one-way ANOVA and Post Hoc Tukey HSD test. Results. Cytotoxicity tests revealed that unpolymerized APL was more cytotoxic compared to ASB after 20 s (P<0.05). By increasing the time to 5 min and 24 h, ASB was more cytotoxic than APL with lower dilutions. Polymerized ASB was more toxic than APL. Conclusion. Both adhesives were cytotoxic in different dilutions, times and curing modes. Cytotoxicity of the unpolymerized self-etching primer (APL) was more than etch-and-rinse adhesive (ASB) in 20 s, which is important clinically and dentists should be aware of the harmful effects and try to minimize it by curing and rinsing soon after composite resin insertion. ASB was more cytotoxic at 5 min and 24h.
背景与目的。本研究旨在评估同一制造商生产的酸蚀冲洗粘结剂和自酸蚀底漆在早期暴露时间的稀释和固化方法对原代人牙龈成纤维细胞细胞毒性的影响。材料与方法。将原代人牙龈成纤维细胞暴露于不同稀释度的Adper Single Bond（ASB）和Adper Prompt L-Pop（APL）（美国3M ESPE公司）。在未聚合模式下分别评估20秒、5分钟和24小时，以及在聚合模式下评估24小时和48小时。使用三种细胞毒性试验（MTT、细胞计数和DNA凝聚）评估细胞毒性。数据采用单因素方差分析和事后Tukey HSD检验进行分析。结果。细胞毒性试验显示，20秒后，未聚合的APL比ASB具有更高的细胞毒性（P<0.05）。随着时间增加到5分钟和24小时，较低稀释度下ASB比APL具有更高的细胞毒性。聚合后的ASB比APL毒性更大。结论。两种粘结剂在不同稀释度、时间和固化模式下均具有细胞毒性。未聚合的自酸蚀底漆（APL）在20秒时的细胞毒性高于酸蚀冲洗粘结剂（ASB），这在临床上具有重要意义，牙医应意识到其有害影响，并在复合树脂充填后尽快通过固化和冲洗将其降至最低。ASB在5分钟和24小时时细胞毒性更大。
