Erdogan Bulent, Bavbek Murad, Sahin Iffet Feride, Caner Hakan, Ozen Ozlem, Denkbas Emir Baki, Altinors Mehmet Nur
Fatih University, Faculty of Medicine, Department of Neurosurgery, and Ministry of Health, Diskapi Educational and Research Hospital, Ankara, Turkey.
Turk Neurosurg. 2010 Jul;20(3):286-94. doi: 10.5137/1019-5149.JTN.3020-10.1.
The objective of this study was to investigate whether the transplantation of fetal umbilical cord tissue cells as a source of stem cells into the acutely injured spinal cord would produce some regenerations and/or functional recovery in a rat model of spinal cord injury.
Five pregnant albino Wistar rats of 12 days gestation were used for obtaining an umbilical cord cell graft. At the second stage of the experiment only Th8-Th9 laminectomy was performed in Group A animals while Group B animals underwent spinal cord hemitransection. The cultured fetal umbilical cord cells coated with Alginate Gel were placed into the lesion cavity immediately after surgery in Group C animals. Group D animals received only Alginate gel sponges into the injured area. All experiment groups were analyzed histologically and immunohistochemically (GFAP, Ki-67, and Pan cadherin) and for motor function after surgery.
The umbilical cord cell transplanted animals showed a significant motor recovery compared to non-transplanted animals at 8 and 21 days after spinal cord injury (p=0.008). Significant GFAP and Ki-67 expressions were noted in transplanted animals (p=0.048) suggesting astroglial proliferation.
Our findings support the possibility of some functional recovery after umbilical cord cell transplantation following spinal cord injury.
本研究的目的是在大鼠脊髓损伤模型中，探讨将胎儿脐带组织细胞作为干细胞来源移植到急性损伤的脊髓中是否会产生一些再生和/或功能恢复。
使用5只妊娠12天的白化Wistar大鼠获取脐带细胞移植物。在实验的第二阶段，A组动物仅进行Th8-Th9椎板切除术，而B组动物进行脊髓半横断术。C组动物在手术后立即将涂有藻酸盐凝胶的培养胎儿脐带细胞放入损伤腔。D组动物仅在受伤区域接受藻酸盐凝胶海绵。所有实验组在术后进行组织学、免疫组织化学（GFAP、Ki-67和泛钙黏着蛋白）分析以及运动功能分析。
与未移植动物相比，脊髓损伤后8天和21天，脐带细胞移植动物显示出显著的运动恢复（p = 0.008）。在移植动物中观察到显著的GFAP和Ki-67表达（p = 0.048），提示星形胶质细胞增殖。
我们的研究结果支持脊髓损伤后脐带细胞移植后出现一些功能恢复的可能性。
