Department of Neurosurgery, Fatih University Faculty of Medicine, Ankara, Turkey.
Neurol Neurochir Pol. 2013 Mar-Apr;47(2):138-44. doi: 10.5114/ninp.2013.33824.
In continuation of our previous experimental study on spinal cord injury (SCI) using fetal stem cells, we investigated here the effects of fetal allogeneic umbilical cord tissue transplant on the urinary bladder morphology in a rat SCI model.
Five pregnant albino Wistar rats at 12 days of gestation were used to obtain the umbilical cord cell graft. In Group 1 (n = 5), Th8-Th9 laminectomy was performed. Group 2 (n = 5) received spinal cord injury. In Group 3 (n = 5), the cultured fetal umbilical cord cells coated with alginate gel were placed into the lesion cavity. In Group 4 (n = 5), only alginate sponges without umbilical cord cells were placed into the injury cavity. The bladders of animals were analyzed pathologically at 21 days after surgery.
The thickness of the epithelium and the lamina propria did not differ among studied groups (p > 0.05). The lamina muscularis thickness was significantly higher in Group 2 and Group 4 than the others (p < 0.05). The bladder weight was similar among Groups 1, 2, and 3 (p > 0.05). Fibrosis was significantly increased in Group 2 (p < 0.05); it was greater in Group 2 than in Group 3 (p < 0.05) but did not differ between Groups 1 and 3 (p > 0.05).
This study suggests that allogeneic umbilical cord tissue transplantation after SCI may prevent bladder wall hypertrophy and fibrosis in the rat SCI model.
在我们之前使用胎儿干细胞进行脊髓损伤（SCI）的实验研究的基础上，我们在此研究了胎儿同种异体脐带组织移植对 SCI 大鼠模型膀胱形态的影响。
使用 5 只妊娠 12 天的白化 Wistar 大鼠获得脐带细胞移植物。在第 1 组（n = 5）中，进行 Th8-Th9 椎板切除术。第 2 组（n = 5）接受脊髓损伤。在第 3 组（n = 5）中，将包被藻酸盐凝胶的培养胎儿脐带细胞置于损伤腔中。在第 4 组（n = 5）中，仅将无脐带细胞的藻酸盐海绵置于损伤腔中。术后 21 天分析动物的膀胱进行病理学分析。
研究各组间上皮和固有层的厚度无差异（p > 0.05）。第 2 组和第 4 组的肌层厚度明显高于其他组（p < 0.05）。第 1、2 和 3 组的膀胱重量相似（p > 0.05）。第 2 组的纤维化明显增加（p < 0.05）；第 2 组比第 3 组（p < 0.05）大，但与第 1 组无差异（p > 0.05）。
本研究表明，SCI 后同种异体脐带组织移植可能预防 SCI 大鼠模型膀胱壁肥大和纤维化。
