Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province, College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
J Ethnopharmacol. 2010 Aug 9;130(3):521-8. doi: 10.1016/j.jep.2010.05.039.
The present study was conducted to evaluate the antioxidant, free radical scavenging, hepatoprotective and anti-inflammatory potential of Parathelypteris nipponica (Franch. et Sav.) Ching.
Antioxidant activity of the methanolic extract of Parathelypteris nipponica (Franch. et Sav.) Ching (TMPN) was studied using in vitro and in vivo models, total flavonoids content in TMPN was found to be 262 +/- 5.6 mg/g (w/w). The TMPN exhibited strong antioxidant activity with EC50 values in reductive ability (0.18 +/- 0.02 mg/ml) and ferric thiocyanate (FTC) assay (0.10 +/- 0.01 mg/ml), strong free radical scavenging activity as evidenced by the low EC50 values in DPPH (1,1-diphenyl-2-picrylhydrazyl) (2.00 +/- 0.02 mg/ml), superoxide anion (0.60 +/- 0.05 mg/ml), OH radicals (0.26 +/- 0.03 mg/ml), and hydrogen peroxide (0.45 +/- 0.03 mg/ml) methods. Acute toxicity study revealed that the LD50 value of the extract was more than the dose 2000 mg/kg bodyweight of mice. Hepatoprotective activity of TMPN was determined by the carbon tetrachloride (CCl4)-induced oxidative tissue injury in rat liver, the extract showed significant hepatoprotective activity that was evident by enzymatic examination and histopathological study. In assessing anti-inflammatory activity the carrageenan-induced rat paw oedema test was used, the extract reduced carrageenan-induced rat paw oedema in dose-dependent manner, achieving high degree of anti-inflammatory activity.
This study provides a scientific basis for the ethnomedical claims that Parathelypteris nipponica (Franch. et Sav.) Ching is effective against inflammation and liver injury.
本研究旨在评估蹄盖蕨科 Parathelypteris nipponica（Franch. et Sav.）Ching 的抗氧化、自由基清除、保肝和抗炎潜力。
采用体外和体内模型研究了蹄盖蕨科 Parathelypteris nipponica（Franch. et Sav.）Ching（TMPN）甲醇提取物的抗氧化活性，发现 TMPN 中的总黄酮含量为 262±5.6mg/g（w/w）。TMPN 表现出很强的抗氧化活性，其还原能力的 EC50 值为 0.18±0.02mg/ml，铁氰化钾（FTC）测定法的 EC50 值为 0.10±0.01mg/ml；DPPH（1,1-二苯基-2-苦基肼）法、超氧阴离子法、OH 自由基法和过氧化氢法的 EC50 值均较低，表明其具有很强的自由基清除活性，分别为 2.00±0.02mg/ml、0.60±0.05mg/ml、0.26±0.03mg/ml 和 0.45±0.03mg/ml。急性毒性研究表明，提取物的 LD50 值大于 2000mg/kg 体重的小鼠剂量。TMPN 的保肝活性通过四氯化碳（CCl4）诱导的大鼠肝氧化组织损伤来确定，结果表明，提取物具有显著的保肝活性，这在酶学检查和组织病理学研究中均有明显体现。在评估抗炎活性时，采用角叉菜胶诱导的大鼠足肿胀试验，提取物呈剂量依赖性地减轻角叉菜胶诱导的大鼠足肿胀，达到高度抗炎活性。
本研究为蹄盖蕨科 Parathelypteris nipponica（Franch. et Sav.）Ching 对炎症和肝损伤有效的民族医学说法提供了科学依据。
