Parkinson Charles R, Butler Andrew, Willson Richard J
GlaxoSmithKline Consumer Healthcare, Weybridge, Surrey, UK.
J Clin Dent. 2010;21(2):31-6.
The objective of this study was to evaluate the utility of a novel acid challenge-based dentin disc occlusion model, and to compare the occluding effect and acid resistance exhibited by currently marketed occlusion dentifrices in vitro.
Ninety-six bovine dentin discs were polished and etched in citric acid (6% w/w) for two minutes to provide a smooth dentin surface with patent tubules. The discs were divided into three treatment groups. Each treatment group was brushed (Oral-B Vitality Precision Clean/EB 17 FlexiSoft head) twice a day, for up to four days, with either a strontium acetate dentifrice (Sensodyne Rapid Relief), an arginine-based dentifrice (Colgate Sensitive Pro-Relief), or water. Prior to and between treatments, the dentin samples were stored in human saliva. On days 3 and 4, following dentifrice treatment and incubation in saliva (60 minutes), the samples were subjected to a grapefruit juice challenge. Eight samples from each treatment group were removed from the study on each day and analyzed by scanning electron microscopy (SEM). The SEM images were graded according to a categorical occlusion scale, and the data were analyzed by ANOVA.
The strontium acetate dentifrice occluded dentin tubules significantly better than the negative control (water) on days 1 through 4 (day 4 p < or = 0.0001). The marketed occlusion-based dentifrices demonstrated various degrees of dentin tubule occlusion over the four days. The strontium acetate dentifrice demonstrated significantly better occlusion than a currently marketed arginine-based occlusion dentifrice on day 1 (p = 0.0337), day 2 (p = 0.0021 ), and day 4 (p < or = 0.0001).
An in vitro model has been developed that can differentiate the dentin tubular occlusive effects of currently marketed occlusion dentifrices. Surface analysis reveals that the occluding deposits vary according to product, and that some are more susceptible to acid mediated dissolution.
本研究的目的是评估一种基于新型酸蚀挑战的牙本质盘封闭模型的效用，并比较目前市售防敏感牙膏在体外表现出的封闭效果和耐酸性。
将96个牛牙本质盘进行抛光，然后在柠檬酸（6% w/w）中蚀刻两分钟，以提供具有开放小管的光滑牙本质表面。将这些牙本质盘分为三个处理组。每个处理组每天使用（欧乐-B劲爽精准清洁/EB 17柔护刷头）刷牙两次，持续四天，分别使用醋酸锶牙膏（舒适达速效抗敏牙膏）、精氨酸牙膏（高露洁全效抗敏牙膏）或水。在处理前和处理期间，将牙本质样本储存在人唾液中。在第3天和第4天，在牙膏处理并在唾液中孵育（60分钟）后，对样本进行葡萄柚汁挑战。每天从每个处理组中取出8个样本退出研究，并通过扫描电子显微镜（SEM）进行分析。根据分类封闭量表对SEM图像进行评分，并通过方差分析对数据进行分析。
在第1天至第4天，醋酸锶牙膏封闭牙本质小管的效果明显优于阴性对照（水）（第4天p≤0.0001）。市售的防敏感牙膏在四天内表现出不同程度的牙本质小管封闭。在第1天（p = 0.0337）、第2天（p = 0.0021）和第4天（p≤0.0001），醋酸锶牙膏的封闭效果明显优于目前市售的精氨酸防敏感牙膏。
已开发出一种体外模型，可区分目前市售防敏感牙膏对牙本质小管的封闭效果。表面分析表明，封闭沉积物因产品而异，且有些更容易受到酸介导的溶解作用影响。
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