Radiation Biology and Health Sciences Division, Bhabha Atomic Research Center, Mumbai, India.
Int J Radiat Biol. 2010 Sep;86(9):719-31. doi: 10.3109/09553002.2010.484480.
An ethanolic extract of Nigella sativa L. (EE-NS) was investigated for its antioxidant properties and radioprotective effects against gamma-radiation-induced oxidative damage.
The radical scavenging activity of the extract was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), deoxyribose degradation and plasmid relaxation assays in a cell-free system. DNA damage studies were performed using a single cell gel electrophoresis (SCGE) assay and micronuclei (MN) formation. Moreover, the alterations in lipid peroxidation and antioxidant enzymes were measured by biochemical methods.
EE-NS showed significant free radical scavenging and protection against DNA damage in cell free systems. Ex vivo treatment of mouse splenic lymphocytes with an ethanolic extract of N. sativa 1 h prior to irradiation (2 Gy) showed significant prevention of the formation of lipid-peroxides and intracellular reactive oxygen species (ROS), which correlated with radiation-induced apoptosis. Moreover, radiation-induced DNA damage was significantly prevented in splenocytes pre-treated with EE-NS. Swiss albino mice fed orally with the different doses of EE-NS (0-100 mg/kg bw) for five consecutive days followed by 2 Gy whole body irradiation (WBI) showed significant protection against oxidative injury to spleen and liver as measured by lipid peroxidation and the activity of antioxidant enzymes. These results were correlated with the prevention of DNA damage as measured by bone marrow micronuclei assay. Our results suggest that oral feeding of extract resulted in increased survival in mice exposed to WBI (7.5 Gy).
The results obtained from the different experimental systems suggest the radioprotective ability of EE-NS involving prevention of radiation-induced oxidative damage.
研究黑种草（EE-NS）的乙醇提取物的抗氧化特性及其对γ 射线诱导的氧化损伤的放射防护作用。
在无细胞体系中，通过 2,2-二苯基-1-苦肼基（DPPH）、脱氧核糖降解和质粒松弛试验来测量提取物的自由基清除活性。使用单细胞凝胶电泳（SCGE）试验和微核（MN）形成来进行 DNA 损伤研究。此外，通过生化方法测量脂质过氧化和抗氧化酶的变化。
EE-NS 在无细胞体系中表现出显著的自由基清除和对 DNA 损伤的保护作用。在照射（2 Gy）前 1 小时用黑种草乙醇提取物对小鼠脾淋巴细胞进行离体处理，显示出对脂质过氧化物和细胞内活性氧（ROS）形成的显著预防作用，这与辐射诱导的细胞凋亡有关。此外，EE-NS 预处理可显著预防脾细胞中辐射诱导的 DNA 损伤。连续 5 天口服不同剂量的 EE-NS（0-100mg/kg bw），然后进行全身 2 Gy 照射（WBI）的瑞士白化小鼠，在脾和肝的氧化损伤方面表现出显著的保护作用，如脂质过氧化和抗氧化酶的活性所示。这些结果与骨髓微核试验中测量的 DNA 损伤预防相关。我们的结果表明，口服提取物可增加暴露于 WBI（7.5 Gy）的小鼠的存活率。
从不同实验系统获得的结果表明 EE-NS 具有放射防护能力，涉及预防辐射诱导的氧化损伤。
