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[GRP78在SPCA-1细胞系对顺铂耐药中的作用]

[The role of GRP78 on the resistance to cisplatin in SPCA-1 cell line].

作者信息

Wu Jing, Wang Qi, Wang Jiarui, Zhang Lichuan, Zhao Long

机构信息

Department of Respiratory Medicine, the Dalian No.4 People Hospital, Dalian 116031, China.

出版信息

Zhongguo Fei Ai Za Zhi. 2010 Jan;13(1):38-41. doi: 10.3779/j.issn.1009-3419.2010.01.07.

DOI:10.3779/j.issn.1009-3419.2010.01.07
PMID:20672702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6000677/
Abstract

BACKGROUND AND OBJECTIVE

The expression levels of glucose-regulated protein 78 (GRP78) were elevated and correlated with resistance to chemotherapy drug VP-16 in lung cancer cells. However, little is known about the relationship between its expression and resistance to cisplatin in lung cancer cells. The aim of this study was to investigate the expression of GRP78 under the induction of A23187 and its significance in the resistance to anti-tumor drugs cisplatin in a human lung cancer SPCA-1 cell line.

METHODS

RT-PCR and Western blot were used to analyze the expression of GRP78 at mRNA and protein levels in SPCA-1 cell line induced byA23187 at different concentrations (0, 1, 2, 4, 6 microM). MTT was used to determine the effect of cisplation on cell survival.

RESULTS

The expressions of GRP78 at both mRNA and protein levels were increased obviously in SPCA-1 cell line induced by A23187, with a manner of dose-dependent of A23187 to a great degree; MTT assay showed that the cell survival rate of the A23187-induced group decreased significantly compare to the control group, also with a concentration-dependent manner of A23187.

CONCLUSION

The expression of GRP78 at both mRNA and protein levels were increased obviously in SPCA-1 cell line induced by A23187. The enhancement of GRP78 showed a negative correlation with the cell survival rate treated by cisplatin. All these indicated that overexpression of GRP78 can enhance the sensitivity to cisplatin and there is correlation between the expression of GRP78 and resistance to cisplatin of human lung cancer SPCA-1 cell line.

摘要

背景与目的

葡萄糖调节蛋白78（GRP78）在肺癌细胞中的表达水平升高，且与对化疗药物依托泊苷（VP-16）的耐药性相关。然而，关于其表达与肺癌细胞对顺铂耐药性之间的关系知之甚少。本研究旨在探讨A23187诱导下人肺癌SPCA-1细胞系中GRP78的表达及其在抗肿瘤药物顺铂耐药中的意义。

方法

采用逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法（Western blot）分析不同浓度（0、1、2、4、6微摩尔）A23187诱导的SPCA-1细胞系中GRP78在mRNA和蛋白质水平的表达。采用噻唑蓝比色法（MTT）测定顺铂对细胞存活的影响。

结果

A23187诱导的SPCA-1细胞系中GRP78在mRNA和蛋白质水平的表达均明显增加，在很大程度上呈A23187剂量依赖性；MTT试验表明，与对照组相比，A23187诱导组的细胞存活率显著降低，也呈A23187浓度依赖性。

结论

A23187诱导的SPCA-1细胞系中GRP78在mRNA和蛋白质水平的表达均明显增加。GRP78的增强与顺铂处理后的细胞存活率呈负相关。所有这些表明，GRP78的过表达可增强对顺铂的敏感性，且GRP78的表达与人肺癌SPCA-1细胞系对顺铂的耐药性之间存在相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d2c/6000677/a9d4ee9d05de/zgfazz-13-1-38-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d2c/6000677/4bc57433c783/zgfazz-13-1-38-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d2c/6000677/c7d24d11f9a5/zgfazz-13-1-38-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d2c/6000677/a9d4ee9d05de/zgfazz-13-1-38-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d2c/6000677/4bc57433c783/zgfazz-13-1-38-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d2c/6000677/c7d24d11f9a5/zgfazz-13-1-38-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d2c/6000677/a9d4ee9d05de/zgfazz-13-1-38-3.jpg

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本文引用的文献

1
Down-regulation of GRP78 is associated with the sensitivity of chemotherapy to VP-16 in small cell lung cancer NCI-H446 cells.葡萄糖调节蛋白78（GRP78）的下调与小细胞肺癌NCI-H446细胞对依托泊苷（VP-16）化疗的敏感性相关。
BMC Cancer. 2008 Dec 17;8:372. doi: 10.1186/1471-2407-8-372.
2
VP-16 resistance in the NCI-H460 human lung cancer cell line is significantly associated with glucose-regulated protein78 (GRP78) induction.NCI-H460人肺癌细胞系中的依托泊苷耐药性与葡萄糖调节蛋白78（GRP78）的诱导显著相关。
Anticancer Res. 2007 Jul-Aug;27(4B):2359-64.
3
Heat shock proteins in cancer: diagnostic, prognostic, predictive, and treatment implications.
癌症中的热休克蛋白：诊断、预后、预测及治疗意义
Cell Stress Chaperones. 2005 Summer;10(2):86-103. doi: 10.1379/csc-99r.1.
4
The glucose-regulated proteins: stress induction and clinical applications.葡萄糖调节蛋白：应激诱导与临床应用
Trends Biochem Sci. 2001 Aug;26(8):504-10. doi: 10.1016/s0968-0004(01)01908-9.
5
Analysis of cellular sensitization with cisplatin-induced apoptosis by glucose-starved stress in cisplatin-sensitive and -resistant A431 cell line.顺铂敏感和耐药A431细胞系中葡萄糖饥饿应激对顺铂诱导细胞凋亡的细胞致敏作用分析
Anticancer Res. 2001 Mar-Apr;21(2A):1029-33.
6
Regulation of SERCA Ca2+ pump expression by cytoplasmic Ca2+ in vascular smooth muscle cells.血管平滑肌细胞中细胞质Ca2+对肌浆网Ca2+泵表达的调节
Am J Physiol Cell Physiol. 2001 Apr;280(4):C843-51. doi: 10.1152/ajpcell.2001.280.4.C843.
7
Down-regulation of the endoplasmic reticulum chaperone GRP78/BiP by vomitoxin (Deoxynivalenol).呕吐毒素（脱氧雪腐镰刀菌烯醇）对内质网伴侣蛋白GRP78/BiP的下调作用
Toxicol Appl Pharmacol. 2000 Feb 1;162(3):207-17. doi: 10.1006/taap.1999.8842.
8
Use of the glucose starvation-inducible glucose-regulated protein 78 promoter in suicide gene therapy of murine fibrosarcoma.
Cancer Res. 1999 Jul 1;59(13):3100-6.
9
Increased sensitivity of human colon cancer cells to DNA cross-linking agents after GRP78 up-regulation.GRP78上调后人类结肠癌细胞对DNA交联剂的敏感性增加。
Biochem Biophys Res Commun. 1999 Apr 13;257(2):361-8. doi: 10.1006/bbrc.1999.0472.
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Down-regulation of epidermal growth factor receptor-signaling pathway by binding of GRP78/BiP to the receptor under glucose-starved stress conditions.在葡萄糖饥饿应激条件下，GRP78/BiP与表皮生长因子受体结合导致该受体信号通路下调。
J Cell Physiol. 1998 Nov;177(2):282-8. doi: 10.1002/(SICI)1097-4652(199811)177:2<282::AID-JCP10>3.0.CO;2-C.